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Journal / Biochemistry / Deteção de fosforilação Rab10 pela atividade…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Deteção de fosforilação Rab10 pela atividade LRRK2 utilizando SDS-PAGE com um Tag de ligação de fosfato
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Deteção de fosforilação Rab10 pela atividade LRRK2 utilizando SDS-PAGE com um Tag de ligação de fosfato

Article DOI: 10.3791/56688-v • 08:55 min • December 14th, 2017
December 14th, 2017 •
1, 1,2
1Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 2Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences, The University of Tokyo

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Summary
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O presente estudo descreve um método simples de detectar níveis endógenos de fosforilação de Rab10 pela rica em leucina repetição quinase 2.

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Bioquímica edição 130 doença de Parkinson rico em leucina repetição quinase 2 Rab10 fosforilação Phos-marca SDS-PAGE immunoblotting
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