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A Drosophila In Vivo Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System

Article DOI: 10.3791/57557-v • 09:55 min • May 5th, 2018
May 5th, 2018 •
*1, *1, 1, 1,2
1Raymond G. Perelman Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, 2Department of Pathology and Laboratory Medicine, University of Pennsylvania
* These authors contributed equally

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Summary
Automatic Translation

Here, we present a protocol using the Drosophila sensory neuron - dendritic arborization (da) neuron injury model, which combines in vivo live imaging, two-photon laser axotomy/dendriotomy, and the powerful fly genetic toolbox, as a platform for screening potential promoters and inhibitors of neuroregeneration.

Tags

Drosophila In Vivo Injury Model Neuroregeneration Peripheral Nervous System Central Nervous System Live Imaging Two-photon Laser Axotomy Dendriotomy Fly Genetic Toolbox Screening Promoters Inhibitors Neurogeneration Candidates Easy Fast Cheap Neurodegenerative Diseases Neuron-glia Interactions Experimental Setups Larvae Collection Culture Bottles
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