Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / DNAzyme-dependent Analysis of rRNA…
JoVE Journal
Developmental Biology

Dieser Inhalt ist Open Access.

JoVE Journal Developmental Biology
DNAzyme-dependent Analysis of rRNA 2’-O-Methylation
Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification
Journal (Developmental Biology)
Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification
Assay for Blood-brain Barrier Integrity in Drosophila melanogaster
Journal (Developmental Biology)
Assay for Blood-brain Barrier Integrity in Drosophila melanogaster
 

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Article DOI: 10.3791/59700-v • 09:12 min • September 16th, 2019
September 16th, 2019 •
1, 1
1School of Biosciences, University of Birmingham

Kapitel

Summary
Automatic Translation

Here we present a protocol for DNAzyme-dependent cleavage of RNA. This enables fast and site-dependent analysis of RNA 2’-O-methylation. This approach can be used for the preliminary or major assessment of snoRNA activity.

Tags

DNAzyme-dependent Analysis RRNA 2'-O-methylation SnoRNAs RNA Methylation DNA Oligonucleotide Molecular Biology Lab DNAzyme Design SnR13-dependent Site Saccharomyces Genome Database Target Gene 10-23 DNAzyme Assay 8-17 DNAzyme Complementary Sequences DNAzyme Catalytic Sequence Yeast Strains RNA Isolation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter