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Journal / Genetics / Identifikation af fodspor af RNA: protein…
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Genetics

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Identifikation af fodspor af RNA
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Identifikation af fodspor af RNA: protein komplekser via RNA Immunopræcipitation i tandem efterfulgt af sekventering (RIPiT-SEQ)

Article DOI: 10.3791/59913-v • 09:26 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1
1Department of Molecular Genetics, Center for RNA Biology, The Ohio State University

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Her præsenterer vi en protokol for at berige endogene RNA-bindingssteder eller "fodspor" af RNA: protein (RNP)-komplekser fra pattedyrsceller. Denne fremgangsmåde involverer to immunoprecipitationer af RNP under enheder og er derfor døbt RNA chromatinimmunpræcipitation i tandem (ripit).

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Genetik RNA-fodaftryk RIPiT RNA-bindingsprotein RBP RNA: protein komplekser RNP
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