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Journal / Genetics / Identificação de pegadas de RNA: complexos…
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Identificação de pegadas de RNA: complexos proteicos via imunoprecipitação de RNA em tandem seguido de sequenciamento (RIPiT-Seq)

Article DOI: 10.3791/59913-v • 09:26 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1
1Department of Molecular Genetics, Center for RNA Biology, The Ohio State University

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Aqui, nós apresentamos um protocolo para enriquecer locais de ligação do RNA endógeno ou "pegadas" de complexos do RNA: proteína (RNP) das pilhas de mamífero. Essa abordagem envolve duas imunoprecipitações de subunidades de RNP e, portanto, é apelidado de imunoprecipitação de RNA em tandem (RIPiT).

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Genética edição 149 pegada do RNA RIPiT proteína RNA-obrigatória RBP RNA: complexos da proteína RNP
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