Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Digital droplet PCR-metode til kvantificering af…
JoVE Journal
Neuroscience
Author Produced

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Neuroscience
Digital droplet PCR-metode til kvantificering af AAV Transduktionseffektivitet i Murine Retina
Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
Journal (Neuroscience)
Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
C. elegans Tracking and Behavioral Measurement
Journal (Neuroscience)
C. elegans Tracking and Behavioral Measurement
 
Click here for the English version

Digital droplet PCR-metode til kvantificering af AAV Transduktionseffektivitet i Murine Retina

Article DOI: 10.3791/63038-v • 09:50 min • December 25th, 2021
December 25th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2
1Molecular Biology, Genetics and Bioengineering Program, Sabanci University, 2Nanotechnology Research and Application Center (SUNUM), Sabanci University

Kapitel

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Denne protokol præsenterer, hvordan man kvantificerer AAV transduktion effektivitet i musen nethinden ved hjælp af digital dråbe PCR (dd-PCR) sammen med lille skala AAV produktion, intravitreal injektion, retinal billeddannelse, og retinal genomisk DNA isolation.

Tags

Neurovidenskab udgave 178
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter