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Journal / Neuroscience / 뮤린 망막에서 AAV 변환 효율의 정량화를위한 디지털 물방울 PCR…
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Neuroscience
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뮤린 망막에서 AAV 변환 효율의 정량화를위한 디지털 물방울 PCR 방법

Article DOI: 10.3791/63038-v • 09:50 min • December 25th, 2021
December 25th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2
1Molecular Biology, Genetics and Bioengineering Program, Sabanci University, 2Nanotechnology Research and Application Center (SUNUM), Sabanci University

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이 프로토콜은 작은 규모의 AAV 생산, 인트라비탈 주사, 망막 이미징 및 망막 게놈 DNA 격리와 함께 디지털 액적 PCR (dd-PCR)을 사용하여 마우스 망막에서 AAV 변환 효율을 정량화하는 방법을 제시합니다.

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신경과학 문제 178
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