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Journal / Biochemistry / 필 블롯 기법: 다층 또는 농축된 생물학적 시료에서 단층을 분리하는 Cryo-EM 시료…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
필 블롯 기법
Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
Journal (Biochemistry)
Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones
Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
Journal (Biochemistry)
Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
 
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필 블롯 기법: 다층 또는 농축된 생물학적 시료에서 단층을 분리하는 Cryo-EM 시료 전처리 방법

Article DOI: 10.3791/64099-v • 07:27 min • June 29th, 2022
June 29th, 2022 •
*1,2, *1, 1,3
1School of Biological Sciences, Georgia Institute of Technology, 2Department of Structural Biology, Genentech, 3School of Chemistry and Biochemistry, Georgia Institute of Technology
* These authors contributed equally

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필 블롯 기술은 다층 및 농축된 생물학적 샘플을 단일 레이어로 분리하여 두께를 줄이고 샘플 농도를 높이며 이미지 처리를 용이하게 하는 Cryo-EM 그리드 준비 방법입니다.

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생화학 184 호
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