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Journal / Bioengineering / 利用线性DNA模板从核酸外切酶缺陷细胞提取物中合成无细胞蛋白…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
利用线性DNA模板从核酸外切酶缺陷细胞提取物中合成无细胞蛋白
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利用线性DNA模板从核酸外切酶缺陷细胞提取物中合成无细胞蛋白

Article DOI: 10.3791/64236-v • 08:11 min • August 9th, 2022
August 9th, 2022 •
*1, *1, 1, 2,3, 4, 1
1INRAe, AgroParisTech, Micalis Institute, Université Paris-Saclay, 2Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 3Medical Faculty, University of Würzburg, 4Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, University of Montpellier
* These authors contributed equally

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Summary
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这里介绍的是用于制备和缓冲液校准大 肠杆菌 BL21 Rosetta2（ΔrecBCD 和 ΔrecB）核酸外切酶 V 敲除菌株细胞提取物的方案。这是一种使用线性DNA模板在无细胞蛋白质合成系统中表达的快速、简单和直接的方法。

Tags

生物工程，第186期，
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