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Journal / Bioengineering / Microinjection Wound Assay and In vivo…
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Bioengineering

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JoVE Journal Bioengineering
Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.
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Microinjection Wound Assay and In vivo Localization of Epidermal Wound Response Reporters in Drosophila Embryos.

Article DOI: 10.3791/50750-v • 11:14 min • November 1st, 2013
November 1st, 2013 •
1, 2, 2, 2
1Sophie Davis School of Biomedical Education, The City College of New York, 2Cell & Developmental Biology, University of California, San Diego

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Summary
Automatic Translation

The embryonic epidermis of very late stage Drosophila embryos provides an in vivo system for rapid puncture wound response analysis and can be combined with genetic manipulations or chemical microinjection treatments to advance studies in wound healing for translation into mammalian models.

Tags

Microinjection Wound Assay In Vivo Localization Epidermal Wound Response Reporters Drosophila Embryos Puncture Injury Glass Needles Epidermal Wound Response Wound Transcriptional Reporters Localized Reporter Signal Living Embryos Larvae Recruitment Of Hemocytes Severe Puncture Injury Late Stage Embryos Embryonic Development Oil Medium Leakage Of Hemolymph Micromanipulation Agar Plates Microscope Slides Genetic Requirements Biological Functions Wound Healing Chemical Compounds Model Organism
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