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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
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Genetik
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JoVE Journal Genetik
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
DOI:

07:55 min

September 11, 2022

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Kapitel

  • 00:05Introduction
  • 00:50DLC Assay
  • 01:54Cell Spheroplasting, Lysis, and Restriction Site Restoration
  • 03:19Restriction Enzyme Digest and Intramolecular Ligation
  • 05:15Results: DLC and DLE Assay Analysis of D-Loops After DSB Induction
  • 07:02Conclusion

Summary

Automatische Übersetzung

The D-loop capture (DLC) and D-loop extension (DLE) assays utilize the principle of proximity ligation together with quantitative PCR to quantify D-loop formation, D-loop extension, and product formation at the site of an inducible double-stranded break in Saccharomyces cerevisiae.

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