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JoVE Journal Genetics
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Article DOI: 10.3791/64240-v • 07:55 min • September 11th, 2022
September 11th, 2022 •
1, 2, 2, 1,3
1Department of Microbiology & Molecular Genetics, University of California, Davis, 2Laboratory of Biology & Modeling of the Cell, École Normale Supérieure de Lyon, 3Department of Molecular & Cellular Biology, University of California, Davis

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The D-loop capture (DLC) and D-loop extension (DLE) assays utilize the principle of proximity ligation together with quantitative PCR to quantify D-loop formation, D-loop extension, and product formation at the site of an inducible double-stranded break in Saccharomyces cerevisiae.

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Homologous Recombination Intermediates Proximity Ligation Quantitative PCR D-loop Capture Assay D-loop Extension Assay Mitotically Growing Yeast Cells Viability Independent Technique BIR Products Key Proteins BRCA2 Bloom And Warner Kilo Cases Centrifugation Psoralen Solution Crosslinking Control UV Light Source Cross-linking Samples 365 Nanometer Long Wave Bulbs
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