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Journal / Immunology and Infection / Peptit-Poloksamin Nanopartikülleri Kullanılarak…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Peptit-Poloksamin Nanopartikülleri Kullanılarak Kültürlenmiş Hücrelerde In vitro Transkribe mRNA'nın Verimli Transfeksiyonu
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Peptit-Poloksamin Nanopartikülleri Kullanılarak Kültürlenmiş Hücrelerde In vitro Transkribe mRNA'nın Verimli Transfeksiyonu

Article DOI: 10.3791/64288-v • 10:16 min • August 17th, 2022
August 17th, 2022 •
*1, *1, 1, 1, 1, 1, 1, 2, 1, 3, 1
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, Third Military Medical University, 2Department of Pediatrics, Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital, Third Military Medical University
* These authors contributed equally

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Kendinden montajlı bir peptid-poloksamin nanopartikülü (PP-sNp), in vitro transkribe edilmiş mesajcı RNA'yı kapsüllemek ve iletmek için mikroakışkan bir karıştırma cihazı kullanılarak geliştirilmiştir. Tanımlanan mRNA / PP-sNp, in vitro kültürlenmiş hücreleri verimli bir şekilde transfekt edebilir.

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İmmünoloji ve Enfeksiyon Sayı 186 Gen dağıtımı in vitro transkribe mesajcı RNA dağıtım sistemleri poloksamin nanopartikül
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