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Enregistrement du courant de potassium voltage-dépendant sur les cardiomyocytes H9C2 via la technique de patch-clamp à cellules entières…
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Enregistrement du courant de potassium voltage-dépendant sur les cardiomyocytes H9C2 via la technique de patch-clamp à cellules entières
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Article
DOI:
10.3791/64805-v
•
08:11 min
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November 11th, 2022
Enregistrement du courant de potassium voltage-dépendant sur les cardiomyocytes H9C2 via la technique de patch-clamp à cellules entières
November 11th, 2022
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Hong Jiang*1, Yating Zhang*1, Ya Hou1, Lijun Li2, Sanyin Zhang3,4, Yi Zhang5, Xianli Meng1,3,4, Xiaobo Wang4
1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 2Chengdu Techman Instrument Co., Ltd., 3Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 4Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine, 5School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine
* These authors contributed equally
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Summary
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Le présent protocole décrit une méthode efficace pour l’acquisition dynamique et en temps réel des courants des canaux potassiques voltage-dépendants (Kv) dans les cardiomyocytes H9c2 en utilisant la technique de patch-clamp à cellules entières.