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Spenningsavhengig kaliumstrømregistrering på9c2-kardiomyocytter via helcelleplaster-klemmeteknikken…
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Medicine
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Spenningsavhengig kaliumstrømregistrering på H9c2-kardiomyocytter via helcelleplaster-klemmeteknikken
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Article
DOI:
10.3791/64805-v
•
08:11 min
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November 11th, 2022
Spenningsavhengig kaliumstrømregistrering på H9c2-kardiomyocytter via helcelleplaster-klemmeteknikken
November 11th, 2022
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Hong Jiang*1, Yating Zhang*1, Ya Hou1, Lijun Li2, Sanyin Zhang3,4, Yi Zhang5, Xianli Meng1,3,4, Xiaobo Wang4
1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 2Chengdu Techman Instrument Co., Ltd., 3Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 4Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine, 5School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine
* These authors contributed equally
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Summary
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Denne protokollen beskriver en effektiv metode for sanntids og dynamisk oppkjøp av spenningsstyrte kalium (Kv) kanalstrømmer i H9c2 kardiomyocytter ved bruk av helcellepatch-klemmeteknikken.