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Registro de corriente de potasio dependiente del voltaje en cardiomiocitos H9c2 mediante la técnica de pinza de parche de células enteras…
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Registro de corriente de potasio dependiente del voltaje en cardiomiocitos H9c2 mediante la técnica de pinza de parche de células enteras
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Article
DOI:
10.3791/64805-v
•
08:11 min
•
November 11th, 2022
Registro de corriente de potasio dependiente del voltaje en cardiomiocitos H9c2 mediante la técnica de pinza de parche de células enteras
November 11th, 2022
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Hong Jiang*1, Yating Zhang*1, Ya Hou1, Lijun Li2, Sanyin Zhang3,4, Yi Zhang5, Xianli Meng1,3,4, Xiaobo Wang4
1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 2Chengdu Techman Instrument Co., Ltd., 3Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 4Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine, 5School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine
* These authors contributed equally
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Summary
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El presente protocolo describe un método eficiente para la adquisición dinámica y en tiempo real de corrientes de canal de potasio (Kv) dependientes de voltaje en cardiomiocitos H9c2 utilizando la técnica de pinza de parche de células enteras.