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Registro de Corrente de Potássio Dependente deVoltagem em Cardiomiócitos H9c2 através da Técnica de Patch-Clamp de Células Inteiras…
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Registro de Corrente de Potássio Dependente de Voltagem em Cardiomiócitos H9c2 através da Técnica de Patch-Clamp de Células Inteiras
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Article
DOI:
10.3791/64805-v
•
08:11 min
•
November 11th, 2022
Registro de Corrente de Potássio Dependente de Voltagem em Cardiomiócitos H9c2 através da Técnica de Patch-Clamp de Células Inteiras
November 11th, 2022
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Hong Jiang*1, Yating Zhang*1, Ya Hou1, Lijun Li2, Sanyin Zhang3,4, Yi Zhang5, Xianli Meng1,3,4, Xiaobo Wang4
1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, 2Chengdu Techman Instrument Co., Ltd., 3Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 4Research Institute of Integrated TCM & Western Medicine, Chengdu University of Traditional Chinese Medicine, 5School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine
* These authors contributed equally
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Summary
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O presente protocolo descreve um método eficiente para a aquisição dinâmica e em tempo real de correntes de canal de potássio dependentes de voltagem (Kv) em cardiomiócitos H9c2 usando a técnica de patch-clamp de células inteiras.