Encyclopedia Of Experiments

Genome Editing Techniques

This collection features techniques used for genome editing in cultured bacterial, animal, and plant cells and in vivo animal models. These methods highlight the applications of CRISPR technology, Zinc finger nucleases, TALENs, transposition and recombination events, shRNA, siRNA, and vector-based transformations to achieve gene knock-in, gene knock-out, and gene regulations.

Encyclopedia Of Experiments

Genome Editing Techniques

Sections

Gene knock-in

Gene knock-out

Gene regulation

Gene knock-in

Gene knock-out

Gene regulation

Gene knock-in

All-in-One CRISPR Genome Editing

A Method for Homology Directed Repair-Based Gene Knock-In in Cultured Cells Using CRISPR-Cas9 System

CRISPR-Mediated Base Editing Tools

A Genome Editing Technique to Induce Targeted Base Substitution

Zinc Finger Nuclease-Based Genome Editing

A Technique for Modifying Genome in Human Pluripotent Stem Cells by Double-Stranded Homology Dependant Repair Mechanism

TALEN-Mediated Targeted Gene Integration

Using Engineered Sequence-Specific Nucleases for the Precise Insertion of Fluorescent Protein Gene into hiPSCs

phiC31-Integrase-Mediated Site-Directed Transgene Integration

A Microinjection Technique for Site-Specific Transgene Integration into an Anopheles Vector

Binary Bacterial Artificial Chromosome Vector Based Gene Transformation in Arabidopsis Plants

A Technique to Generate Transgenic Plants With Single-Copy Insertion

Tol2 Transposon-Mediated Zebrafish Transgenesis

A Procedure to Generate Transgenic Zebrafish Following the Coinjection of Tol2 System Into Fertilized Zebrafish Embryos

PiggyBac Transposon-Mediated Gene Editing in Human iPSCs

A Procedure to Integrate Gene of Interest in Human iPSCs Using PiggyBac Transposon System

Gene knock-out

CRISPR Concatemer-Mediated Multiple Gene Knockout

A Technique to Simultaneously Knockout Multiple Genes by Non-Homologous End-Joining Pathway in Mouse Intestinal Cells

CRISPR Interference-Based Gene Silencing

A Technique for Targeted Repression of Gene Function in Pathogenic Leptospira

CreER-LoxP System-Based Target Gene Inactivation

A Tamoxifen-Inducible Cre Recombinase System for Target Gene Knockout in a Mouse Model

Gene regulation

Short Hairpin RNA-Mediated Gene Knockdown in iHSPCs In Vitro

A Lentivirus-Based shRNA Expression System Delivery into iHSPCs for Knockdown of Specific Gene Expression

Inducible Tet-On Regulatable System-Based Gene Expression In Vivo

A Tetracycline-Induced Gene Expression System for Target Gene Transcription Activation in Mouse Model

siRNA Reverse Transfection-Based Gene Silencing In Vitro

A Procedure to Deliver Small Interfering RNA in Cultured Cells to Inactivate Target Gene Expression

Inducible Tet-Off Regulatable System

An In Vitro Method to Modulate Gene Expression in Cultured Cells Using Tetracycline-Off System

Morpholino Mediated Gene Editing

A Gene Knockdown Technique Using Translational Blocking Morpholino Oligonucleotides in Single-Celled Cavefish Eggs