Encyclopedia Of Experiments

Electrophoresis Techniques

This collection highlights the mechanisms behind various electrophoresis techniques to separate biomolecules such as DNA, RNA, peptides, individual proteins, protein complexes, and amyloids. These methods utilize variants of agarose gel- or polyacrylamide gel-based matrices to separate biomolecules under the influence of an electric field.

Encyclopedia Of Experiments

Electrophoresis Techniques

Sections

Electrophoresis Techniques

Polyacrylamide Gel Electrophoresis

An Analytical Technique to Detect Heparan Sulfates of Varying Chain Lengths Isolated from Mouse Lung Tissue

Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis for Protein Analysis

A Technique to Separate and Visualize Proteins Based on Molecular Weight

Denaturing Urea Polyacrylamide Gel Electrophoresis for RNA Analysis

A Technique to Separate Fluorescently Labeled Phosphorylated RNA Oligonucleotides from their Non-Phosphorylated Equivalents

Two-Dimensional Semi-Denaturing Agarose Gel Electrophoresis

A Technique to Separate Polymorphic Amyloids Fibers Based on Size Heterogeneity

Blue Native Polyacrylamide Gel Electrophoresis

A Non-Denaturing Separation Technique for Analysis of Intact Mitochondrial Respiratory Chain Complexes

Multimer-PAGE for Separating Native Protein Complexes

A Hybrid Separation Technique Consisting of Blue Native-PAGE and SDS-PAGE to Separate Intact Multimeric Proteins From Tissue Lysate

Agarose Gel Electrophoresis of DNA Amplicons Post PCR

A Method to Analyze Products of Multiplex PCR and Evaluate PCR Reaction Success

Capillary Electrophoresis

A Technique to Analyze Fluorescently Labeled Polymerase Chain Reaction Amplicons

Alkaline Single Cell Gel Electrophoresis

A Sensitive Technique for Quantitative Estimation of DNA Damage in Individual Cancer Cells Following Chemotherapy

Microfluidic Capillary Electrophoresis for Fragment Sizing of PCR Products

A Microchip-Based Electrophoretic Separation Technique to Separate DNA Fragments Based on Size

Non-Reducing SDS PAGE

A Method to Analyze Disulfide-Linked Multimeric Protein Complexes

Liquid Phase Isoelectric Focusing

An Electrophoresis Technique to Separate Bioactive Molecules from Crude Gymnema sylvestre Extract

Mobility Shift Affinity Capillary Electrophoresis

A Method to Analyze Sample-Ligand Interactions Depending on Differential Migration of Protein-Ligand Complexes

Two-Dimensional Polyacrylamide Gel Electrophoresis

A Separation Technique for Analysis of Proteins in Complex Protein Mixture Based on Charge and Molecular Weight

Zymography Gel Electrophoresis

An Electrophoretic Technique to Detect Matrix Metalloproteinases by Assessing the Enzymatic Activity