Encyclopedia Of Experiments

PCR Techniques

The focus of this collection extends to a range of applications and methods that utilize Polymerase Chain Reaction (PCR) to identify and examine genetic markers, mutations, and sequences. These techniques encompass mutation detection, annealing temperature optimization, genetic marker quantification, infection diagnosis, enumeration of infective particles, nucleic acid amplification, analysis of epigenetic modifications, and detection of transcript variants.

Encyclopedia Of Experiments

PCR Techniques

Sections

PCR Techniques

Digital Droplet Polymerase Chain Reaction for Indel Mutation Detection in Target Genes

Gradient Polymerase Chain Reaction to Determine the Optimum Annealing Temperature

Duplex Digital PCR for Simultaneous Quantification of Dual Genetic Markers

Real-Time Quantitative Reverse Transcription PCR for Diagnosing Viral Infections

Wild-Type Blocking PCR to Detect Low-Frequency Somatic Mutations

Microsphere Polymerase Chain Reaction to Amplify Single-Stranded DNA

Nested-PCR to Detect a Specific Viral Genomic Sequence

High-Resolution Melting PCR to Determine Sequence Variations in Mutant DNA Sequences

Multiplex Droplet Polymerase Chain Reaction to Detect DNA Methylation in Leukocytes

Chip-Based Digital PCR to Detect Rare Transcript Variants Using a Nanofluidic Chip

Quantitative Polymerase Chain Reaction to Enumerate Bacteriophages

Chip-in-a-Tube-Based Digital PCR for Quantification of Single Nucleotide Variants