Encyclopedia Of Experiments

Biomolecular Interaction Detection Techniques

This collection features various methods and techniques used to study protein-protein interactions. These approaches encompass scientific tools and technologies, such as biosensors, imaging techniques, assays, and spectroscopy, that provide insights into the intricate world of protein interactions and their roles in biological systems, enabling a deeper understanding of the events that transpire at the molecular level. These interactions are fundamental in numerous cellular activities, including signal transduction, enzymatic reactions, gene regulation, and structural organization.

Encyclopedia Of Experiments

Biomolecular Interaction Detection Techniques

This collection features various methods and techniques used to study protein-protein interactions. These approaches encompass scientific tools and technologies, such as biosensors, imaging techniques, assays, and spectroscopy, that provide insights into the intricate world of protein interactions and their roles in biological systems, enabling a deeper understanding of the events that transpire at the molecular level. These interactions are fundamental in numerous cellular activities, including signal transduction, enzymatic reactions, gene regulation, and structural organization.

Sections

Protein-protein interactions

Protein-nucleic acid interactions

Protein-lipid interactions

Protein-protein interactions

Protein-nucleic acid interactions

Protein-lipid interactions

Protein-protein interactions

Graphene Biosensor-Based Field-Effect Biosensing to Detect Protein-Protein Interactions

FLIM-FRET Imaging for Characterization of Protein-Protein Interactions in Live Bacteria

Cell Aggregation Assay to Detect Trans Interactions between Cell Adhesion Molecules

Proximity Ligation Assay to Study In Situ Protein-Protein Interactions

On-Membrane Protein Digestion to Prepare Co-Immunoprecipitated Proteins for Interaction Studies

Biolayer Interferometry Technology to Detect Interactions between Ligand and Analyte

Fluorescence Fluctuation Spectroscopy to Study Protein Interaction at Cell Contacts

Immunoprecipitation Assay to Study Enzyme Receptor Interactions in Transfected Cells

ELISA-Based Method to Analyze Interactions between Bait and Prey Proteins

β-Lactamase-Based Conductimetric Biosensor Assay for Protein-Protein Interactions

Biotinylated Cell-Penetrating Peptide Probe to Detect Protein-Protein Interactions

Yeast Two-Hybrid Assay to Determine Protein Self-Association in Yeast Cells

Tandem Affinity Purification Assay to Study Protein-Protein Interactions

Surface Plasmon Resonance to Study Biomolecular Interactions Using a Sensor Chip

Nuclear Magnetic Resonance to Study Atomic Level Protein-Protein Interactions

Fluorescence Fluctuation Spectroscopy to Study Protein Homo-Oligomerization

Bimolecular Fluorescence Complementation-Coupled Photoactivated Localization Microscopy

Fluorescence Anisotropy-Based Detection of Protein-Protein Interactions

Mass Photometry to Study Antigen-Antibody Interactions on a Single-Molecule Level

Using Phage Display to Select Proteins with High Affinity to a Target Protein

Quantitative In-Cell Hydrogen NMR Spectroscopy to Monitor Protein-Ligand Interactions

Quartz-Crystal Microbalance Biosensor-Based Biopanning to Study Protein-Drug Interactions

Size-Exclusion Chromatography with Multi-Angle Light Scattering for Protein Oligomers

Bimolecular Complementation Affinity Purification to Isolate Two Interacting Proteins

Competitive Binding Assay to Identify Compounds Disrupting Receptor-Ligand Interactions

ELISA-Based Reporter Assay to Study the Receptor-Ligand Interaction in BW Cells

Biomembrane Force Probe to Quantitate Receptor-Ligand Interactions

Competition Binding Assay to Study Competing GTPase-Binding Protein Partners

Viral Antigen Microarray Assay to Detect Antibody Isotypes in Serum Against Viral Antigen

Protein-nucleic acid interactions

Electrophoretic Mobility Shift Assay for Detection of Transcription Factor-DNA Complexes

Chromatin Immunoprecipitation to Identify Target Protein Binding Sites on Genomic DNA

Fluorescence Anisotropy to Determine Transcription Factor-DNA Binding Affinity

Reporter Gene Repression Assay to Study Translational Regulation of a Target Gene

Modified Yeast-One Hybrid Assay to Detect Heteromeric Protein Complex-DNA Interactions

Helicase Activity Measurement of a Target Protein Using Biotin-Labeled RNA Duplexes

Immunofluorescence-Based Visualization of DNA Repair Protein Interactions

RNA-Protein Pull-Down Assay to Isolate RNA-Binding Proteins via Affinity Extraction

SELEX-Based In Vitro Binding Assay to Identify RNA-Protein Interactions

Single-Molecule Imaging to Visualize Fusion Protein Condensates Assembly on DNA Curtains

Differential Scanning Calorimetry to Study DNA Aptamer-Thermolabile Ligand Interaction

Protein-lipid interactions

Microscale Thermophoresis to Study Protein-Lipid Interactions in Solution

Quantitative Flow Cytometry to Study Labeled Protein-Phospholipid Vesicle Interactions

Dissipative Microgravimetry Technique to Study Protein-Lipid Bilayer Interaction

pH Modulation Assay on Supported Lipid Bilayers to Detect Protein-Phosphoinositide Interactions