JoVE Encyclopedia of Experiments

Immunology

Video encyclopedias of advanced research experiments for scientists in academia and biotech

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1,250+ Videos

Filmed at top university labs

Practice and theory for each experiment

Table of Contents

Immune Systems and Components

45 Videos

194 Minutes

Cell Isolation and Culture

20 Videos

82 Minutes

Isolation of Monocytes from Whole Blood by Immunomagnetic Negative Selection
4:27

Isolation of Monocytes from Whole Blood by Immunomagnetic Negative Selection

1.5K views

Isolation and Culture of Mouse Kupffer Cells
3:53

Isolation and Culture of Mouse Kupffer Cells

3.1K views

Isolation of Macrophages from Mouse Dorsal Root Ganglion Using Mechanical Dissociation
6:56

Isolation of Macrophages from Mouse Dorsal Root Ganglion Using Mechanical Dissociation

770 views

Isolation of Lymphocytes from a Mouse Peyer's Patch
6:02

Isolation of Lymphocytes from a Mouse Peyer's Patch

1.4K views

Isolation of Peripheral Blood Mononuclear Cells from Human Buffy Coats
2:45

Isolation of Peripheral Blood Mononuclear Cells from Human Buffy Coats

2.6K views

Isolation and Purification of B Cells from Human Peripheral Blood
3:58

Isolation and Purification of B Cells from Human Peripheral Blood

570 views

Isolation and Culture of Bone Marrow Stromal Cells from a Mouse Tibia
4:00

Isolation and Culture of Bone Marrow Stromal Cells from a Mouse Tibia

1.2K views

Flotation-Based T Cell Isolation Using Buoyancy-Activated Cell Sorting
3:18

Flotation-Based T Cell Isolation Using Buoyancy-Activated Cell Sorting

568 views

Culturing of Activated T Cells from Human Peripheral Blood Mononuclear Cells
3:25

Culturing of Activated T Cells from Human Peripheral Blood Mononuclear Cells

765 views

Isolation of Marginating Hepatic Leukocytes from a Mouse Liver
3:42

Isolation of Marginating Hepatic Leukocytes from a Mouse Liver

387 views

Natural Killer Cell Isolation from Liver Tissue for Selective Expansion
4:17

Natural Killer Cell Isolation from Liver Tissue for Selective Expansion

515 views

Ex Vivo Expansion of Natural Killer Cells from Peripheral Blood Mononuclear Cells
3:14

Ex Vivo Expansion of Natural Killer Cells from Peripheral Blood Mononuclear Cells

685 views

Isolation of Immune Regulatory Sertoli Cells from Mouse Seminiferous Tubules
4:25

Isolation of Immune Regulatory Sertoli Cells from Mouse Seminiferous Tubules

513 views

Selective Isolation of  CD4<sup>+</sup> Vδ1<sup>+</sup> γδ T Cells from Human Peripheral Blood Mononuclear Cells
6:39

Selective Isolation of CD4+ Vδ1+ γδ T Cells from Human Peripheral Blood Mononuclear Cells

362 views

Isolation of Cytotoxic Neutrophils from Tumor-Bearing Mouse Blood
4:21

Isolation of Cytotoxic Neutrophils from Tumor-Bearing Mouse Blood

480 views

Isolation of Gingival Immune Cell Network from Murine Teeth Blocks
3:19

Isolation of Gingival Immune Cell Network from Murine Teeth Blocks

461 views

A Technique to Isolate Primary Mast Cells from a Murine Peritoneal Cavity
4:17

A Technique to Isolate Primary Mast Cells from a Murine Peritoneal Cavity

649 views

Magnetic Isolation of Microglia from Mouse Pup Brains
4:45

Magnetic Isolation of Microglia from Mouse Pup Brains

498 views

Harvesting an Inguinal Draining Lymph Node from a Murine Model
2:29

Harvesting an Inguinal Draining Lymph Node from a Murine Model

2.9K views

A Dissection Method to Harvest Murine Popliteal Draining Lymph Nodes for Ex Vivo Imaging
2:16

A Dissection Method to Harvest Murine Popliteal Draining Lymph Nodes for Ex Vivo Imaging

2.3K views

Immune Response

45 Videos

176 Minutes

Host Defense Mechanism

24 Videos

95 Minutes

Monitoring Neutrophil Recruitment Dynamics and Bacterial Burden at the Infection Site in a Murine Model
4:29

Monitoring Neutrophil Recruitment Dynamics and Bacterial Burden at the Infection Site in a Murine Model

689 views

Activation of Mouse Bone Marrow-Derived Macrophages in Response to Antibodies
6:51

Activation of Mouse Bone Marrow-Derived Macrophages in Response to Antibodies

422 views

In Vivo Activation of Peritoneal Macrophages in Response to Antibodies in a Murine Model
5:05

In Vivo Activation of Peritoneal Macrophages in Response to Antibodies in a Murine Model

476 views

An Ex Vivo Technique to Induce Neuronal Death via Oligodendrocyte-Specific CD8+ T Cells
3:50

An Ex Vivo Technique to Induce Neuronal Death via Oligodendrocyte-Specific CD8+ T Cells

300 views

Evaluation of Vaccine-Induced Immunity Against Bacterial Infection in a Mouse Model
8:18

Evaluation of Vaccine-Induced Immunity Against Bacterial Infection in a Mouse Model

480 views

Organelle Dynamics in B Lymphocytes following Activation with Antigen-Coated Beads
3:24

Organelle Dynamics in B Lymphocytes following Activation with Antigen-Coated Beads

271 views

B Cell Activation on an Antigen-Coated Coverslip
2:41

B Cell Activation on an Antigen-Coated Coverslip

474 views

A Technique to Generate a Murine Model of Dextran Sulfate Sodium-Induced Colitis
3:20

A Technique to Generate a Murine Model of Dextran Sulfate Sodium-Induced Colitis

458 views

Effect of the Anti C-fms Antibody on Osteoclastogenesis in Mouse Bone Marrow Cells In Vitro
4:27

Effect of the Anti C-fms Antibody on Osteoclastogenesis in Mouse Bone Marrow Cells In Vitro

258 views

Quantification of Inflammatory Mediators in Infected Human Tissue-Derived Cells
3:45

Quantification of Inflammatory Mediators in Infected Human Tissue-Derived Cells

320 views

Determination of ROS Production by Neutrophils upon Interaction with Opsonized Biofilm
3:47

Determination of ROS Production by Neutrophils upon Interaction with Opsonized Biofilm

325 views

An In Vitro Assay to Study the Interaction between Neutrophils and Biofilm
3:06

An In Vitro Assay to Study the Interaction between Neutrophils and Biofilm

273 views

Detection of Inflammasome Activation via Fluorescence Microscopy
3:44

Detection of Inflammasome Activation via Fluorescence Microscopy

339 views

An In Vitro Technique to Study Inflammasome Complex Formation in Macrophages
3:06

An In Vitro Technique to Study Inflammasome Complex Formation in Macrophages

296 views

A Lactate Dehydrogenase Release Assay To Detect Macrophage Death Following Nigericin Exposure
3:02

A Lactate Dehydrogenase Release Assay To Detect Macrophage Death Following Nigericin Exposure

416 views

Induction and Visualization of Neutrophil Extracellular Traps in Pre-Labeled Neutrophils
4:32

Induction and Visualization of Neutrophil Extracellular Traps in Pre-Labeled Neutrophils

480 views

Isolation of Lipids from Human Blood-Derived Neutrophils by Biphasic Separation
2:57

Isolation of Lipids from Human Blood-Derived Neutrophils by Biphasic Separation

311 views

A Lactate Dehydrogenase Release Assay to Detect <em>Cryptococcus neoformans</em> Infection in Macrophages
3:25

A Lactate Dehydrogenase Release Assay to Detect Cryptococcus neoformans Infection in Macrophages

316 views

A Permeable Membrane Insert-Based Infection System to Study the Impact of Bacterial Toxins
3:28

A Permeable Membrane Insert-Based Infection System to Study the Impact of Bacterial Toxins

251 views

Extracting Host Cell Lysate from Infected Host Cells Cultured in a Permeable Membrane System
4:15

Extracting Host Cell Lysate from Infected Host Cells Cultured in a Permeable Membrane System

237 views

Detection of Bacterial Adherence to Host Cells Using Fluorescence Imaging
3:05

Detection of Bacterial Adherence to Host Cells Using Fluorescence Imaging

299 views

An Imaging Technique to Study the Calcium Response in Bacteria-Infected Cells
3:43

An Imaging Technique to Study the Calcium Response in Bacteria-Infected Cells

291 views

Analysis of Immune Synapses between Human T Cells and SEB-Loaded Raji Cells using Imaging Flow Cytometry
4:28

Analysis of Immune Synapses between Human T Cells and SEB-Loaded Raji Cells using Imaging Flow Cytometry

331 views

An Assay to Study the Regulation of Macrophage Phagocytosis by Mesenchymal Stem Cells
3:00

An Assay to Study the Regulation of Macrophage Phagocytosis by Mesenchymal Stem Cells

392 views

Immunopathology

46 Videos

154 Minutes

Host-Pathogen Interaction Models

22 Videos

78 Minutes

A Technique to Induce Chronic Experimental Autoimmune Dry Eye in Rats
1:39

A Technique to Induce Chronic Experimental Autoimmune Dry Eye in Rats

197 views

<em>Malassezia</em>-Induced Skin Inflammation and Fungal Burden Analysis in a Murine Model
3:46

Malassezia-Induced Skin Inflammation and Fungal Burden Analysis in a Murine Model

557 views

A Murine Skin Wound Infection Model to Study the Immune Response against a Bacterial Pathogen
4:00

A Murine Skin Wound Infection Model to Study the Immune Response against a Bacterial Pathogen

534 views

A Human Cervical Mucosa Infection Model to Study HIV Pathogenesis in Ex Vivo Conditions
4:51

A Human Cervical Mucosa Infection Model to Study HIV Pathogenesis in Ex Vivo Conditions

339 views

Intravital Microscopy to Analyze Blood Cell-Endothelial Interactions in an Inflammation Mouse Model
4:45

Intravital Microscopy to Analyze Blood Cell-Endothelial Interactions in an Inflammation Mouse Model

464 views

Subcutaneous Injection of Bacterial Antigen to Induce Systemic Inflammation in a Murine Model
3:52

Subcutaneous Injection of Bacterial Antigen to Induce Systemic Inflammation in a Murine Model

684 views

A Murine Model of Primed Mycobacterial Uveitis to Study Post-Infectious Uveitis
4:00

A Murine Model of Primed Mycobacterial Uveitis to Study Post-Infectious Uveitis

331 views

A Technique to Establish a Bacterial Infection Model in Insect Larvae
3:54

A Technique to Establish a Bacterial Infection Model in Insect Larvae

455 views

Measurement of the In Vivo Burden of Bacteria Expressing Luciferase in an Insect Larva
3:23

Measurement of the In Vivo Burden of Bacteria Expressing Luciferase in an Insect Larva

257 views

An Air Pouch Mouse Model for Lipopolysaccharide-Induced Inflammatory Exudate Collection
3:41

An Air Pouch Mouse Model for Lipopolysaccharide-Induced Inflammatory Exudate Collection

1.1K views

A Technique to Generate an Adjuvant-Induced Arthritis Mouse Model
3:29

A Technique to Generate an Adjuvant-Induced Arthritis Mouse Model

1.3K views

An Intragastric Gavage Technique for Controlled <em>Helicobacter</em> Infection in Mice
3:04

An Intragastric Gavage Technique for Controlled Helicobacter Infection in Mice

820 views

Transurethral Catheterization and Bacterial Inoculation to Generate a Urinary Tract Infection Model in Male Mice
2:32

Transurethral Catheterization and Bacterial Inoculation to Generate a Urinary Tract Infection Model in Male Mice

384 views

Establishment of a Urinary Tract Infection Model in Female Mice
2:54

Establishment of a Urinary Tract Infection Model in Female Mice

571 views

Induction of Graft Versus Host Disease in Mouse Models via Allogeneic Bone Marrow and Splenic Cell Transplantation
5:54

Induction of Graft Versus Host Disease in Mouse Models via Allogeneic Bone Marrow and Splenic Cell Transplantation

349 views

Generation of a Mouse Model of Experimental Cerebral Malaria Using Host Mosquito-Derived Sporozoites
3:28

Generation of a Mouse Model of Experimental Cerebral Malaria Using Host Mosquito-Derived Sporozoites

283 views

A Spray Inoculation Technique to Establish Fungal Infection in Rice Plant Leaves
3:30

A Spray Inoculation Technique to Establish Fungal Infection in Rice Plant Leaves

445 views

Establishment of Rice Plant Leaf Infection through Fungal Inoculation via Wounding
2:26

Establishment of Rice Plant Leaf Infection through Fungal Inoculation via Wounding

392 views

Intrabronchial Instillation of <em>Streptococcus pneumoniae</em> in Immunocompetent Rodent Models
4:46

Intrabronchial Instillation of Streptococcus pneumoniae in Immunocompetent Rodent Models

261 views

Establishing a Mouse Model of Pleural Disseminated Lung Cancer
2:36

Establishing a Mouse Model of Pleural Disseminated Lung Cancer

602 views

A Technique to Induce Chronic Experimental Autoimmune Dry Eye in Rats
3:03

A Technique to Induce Chronic Experimental Autoimmune Dry Eye in Rats

0 views

Induction of Experimental Autoimmune Encephalomyelitis in a Mouse Model
2:45

Induction of Experimental Autoimmune Encephalomyelitis in a Mouse Model

556 views

Assay and Analysis

15 Videos

47 Minutes

Immunodiagnostics

162 Videos

683 Minutes

Cellular Immunodiagnostics

46 Videos

192 Minutes

Analysis of Efferocytosis of Apoptotic Thymocytes by Peritoneal Macrophages
5:36

Analysis of Efferocytosis of Apoptotic Thymocytes by Peritoneal Macrophages

762 views

Flow Cytometry Staining of <em>Mycobacterium tuberculosis</em> Infected Macrophage Subpopulations
3:18

Flow Cytometry Staining of Mycobacterium tuberculosis Infected Macrophage Subpopulations

466 views

Bacterial Cell Staining and Preparation for Immunological Studies
2:57

Bacterial Cell Staining and Preparation for Immunological Studies

460 views

Detection of Vitronectin Binding to Bacterial Surfaces using Flow Cytometry
3:05

Detection of Vitronectin Binding to Bacterial Surfaces using Flow Cytometry

284 views

Evaluation of Stromal Cytokine-Induced Intracellular Protein Phosphorylation Using Phospho-Flow Cytometry
3:40

Evaluation of Stromal Cytokine-Induced Intracellular Protein Phosphorylation Using Phospho-Flow Cytometry

342 views

Analyzing Basophil Activation in the Presence of a Test Allergen using Flow Cytometry
5:37

Analyzing Basophil Activation in the Presence of a Test Allergen using Flow Cytometry

360 views

A Technique for Magnetic Glass Particle-Mediated Nucleic Acid Extraction from Blood
3:43

A Technique for Magnetic Glass Particle-Mediated Nucleic Acid Extraction from Blood

425 views

Measuring Mast Cell Exocytosis via Neuropeptide Y Monomeric Red Fluorescent Protein Release
3:14

Measuring Mast Cell Exocytosis via Neuropeptide Y Monomeric Red Fluorescent Protein Release

231 views

Real-Time Monitoring of Energy Metabolism in Human NK Cells Using an Extracellular Flux Analyzer
4:41

Real-Time Monitoring of Energy Metabolism in Human NK Cells Using an Extracellular Flux Analyzer

296 views

Natural Killer Cell-Mediated Cytotoxicity Assay Sample Preparation for Flow Cytometric Analysis
2:40

Natural Killer Cell-Mediated Cytotoxicity Assay Sample Preparation for Flow Cytometric Analysis

373 views

Detection of Rhesus Factor in Human Blood Samples using Magnetic Levitation
4:10

Detection of Rhesus Factor in Human Blood Samples using Magnetic Levitation

299 views

An In Vitro Technique to Study T Cell Interaction with Supported Planar Lipid Bilayers
5:29

An In Vitro Technique to Study T Cell Interaction with Supported Planar Lipid Bilayers

318 views

Effect of an Immunomodulatory Pharmacological Agent on the Interaction Between Dendritic Cells and CD4+ T Cells
2:40

Effect of an Immunomodulatory Pharmacological Agent on the Interaction Between Dendritic Cells and CD4+ T Cells

260 views

Evaluation of Controlled T Cell Activation with a Photoactivatable Peptide MHC
4:54

Evaluation of Controlled T Cell Activation with a Photoactivatable Peptide MHC

275 views

A Technique to Detect Extrapulmonary Tuberculosis via Superparamagnetic Nanoprobes
2:59

A Technique to Detect Extrapulmonary Tuberculosis via Superparamagnetic Nanoprobes

287 views

Monitoring the Arrival of GFP-Tagged Surface Proteins to the Trans-Golgi Network Using Functionalized Nanobodies
5:00

Monitoring the Arrival of GFP-Tagged Surface Proteins to the Trans-Golgi Network Using Functionalized Nanobodies

216 views

A Microfluidic Chip-Based Method for Rapid Neutrophil Chemotaxis Analysis Using Whole Blood
3:54

A Microfluidic Chip-Based Method for Rapid Neutrophil Chemotaxis Analysis Using Whole Blood

275 views

Assessment of the Activation of Multiple Caspases in Macrophages
4:01

Assessment of the Activation of Multiple Caspases in Macrophages

258 views

A Multiplex Bead-Based Assay for Analyzing Cytokines in Human Tear Samples
4:46

A Multiplex Bead-Based Assay for Analyzing Cytokines in Human Tear Samples

340 views

Measuring Alloreactivity in a Mixed Population of T Cells
5:13

Measuring Alloreactivity in a Mixed Population of T Cells

216 views

Identification and Enumeration of Immune Cells in Bronchoalveolar Lavage Fluid by Flow Cytometry
3:03

Identification and Enumeration of Immune Cells in Bronchoalveolar Lavage Fluid by Flow Cytometry

632 views

A Peptide-MHC-I Tetramer Staining Technique to Analyze SIV-Specific CD8<sup>+</sup> Memory T Cells
4:25

A Peptide-MHC-I Tetramer Staining Technique to Analyze SIV-Specific CD8+ Memory T Cells

330 views

A Laminar Flow-Based Assay to Study the Interactions Between Leukocytes and Endothelial Cells
3:54

A Laminar Flow-Based Assay to Study the Interactions Between Leukocytes and Endothelial Cells

332 views

Quantifying Bulk Autophagic Sequestration Activity in Mammalian Cells
4:33

Quantifying Bulk Autophagic Sequestration Activity in Mammalian Cells

185 views

An Assay to Screen Bioactive Nanoparticles for Toll-Like Receptor Signaling Inhibition
3:53

An Assay to Screen Bioactive Nanoparticles for Toll-Like Receptor Signaling Inhibition

244 views

The Effects of Shear Stress on Platelets Treated with Antibodies Against Mechanosensory Receptors
3:11

The Effects of Shear Stress on Platelets Treated with Antibodies Against Mechanosensory Receptors

253 views

Measuring TEER in X-ray-Irradiated Epithelial Cells Co-cultured With PBMCs
2:17

Measuring TEER in X-ray-Irradiated Epithelial Cells Co-cultured With PBMCs

369 views

An In Vitro Fluorescence-Based Assay to Measure Plasma Membrane Resealing Efficiency
4:34

An In Vitro Fluorescence-Based Assay to Measure Plasma Membrane Resealing Efficiency

263 views

An Immunohistochemistry Staining for the Detection of Rabies Virus Antigens
4:34

An Immunohistochemistry Staining for the Detection of Rabies Virus Antigens

303 views

Immunohistochemistry to Study the Relationship Between Macrophages and Infiltrated Immune Cells
4:00

Immunohistochemistry to Study the Relationship Between Macrophages and Infiltrated Immune Cells

258 views

An Enhanced Crosslinking Immunoprecipitation Method for Efficient Identification of Protein-bound RNA in Mouse Testes
5:07

An Enhanced Crosslinking Immunoprecipitation Method for Efficient Identification of Protein-bound RNA in Mouse Testes

273 views

An Assay for Cell Cycle Analysis of Antigen-Specific CD8<sup>+</sup> T Cells
6:20

An Assay for Cell Cycle Analysis of Antigen-Specific CD8+ T Cells

285 views

Measuring Microglial Uptake of Intravitreally-Delivered Fluorescent Particles Using Flow Cytometry
4:34

Measuring Microglial Uptake of Intravitreally-Delivered Fluorescent Particles Using Flow Cytometry

284 views

A Flow Cytometry Assay to Identify Immune Cell Subsets in Peripheral Blood Mononuclear Cells
4:57

A Flow Cytometry Assay to Identify Immune Cell Subsets in Peripheral Blood Mononuclear Cells

773 views

Real-Time Assessment of the Antifungal Activity of Primary Human Immune Cells
2:22

Real-Time Assessment of the Antifungal Activity of Primary Human Immune Cells

212 views

An Assay to Quantify Cytosolic and Vacuolar <em>Salmonella</em> in Infected Primary Macrophages
6:27

An Assay to Quantify Cytosolic and Vacuolar Salmonella in Infected Primary Macrophages

253 views

A Cellular Assay for the Identification of CXCR4-Interacting Agents
5:52

A Cellular Assay for the Identification of CXCR4-Interacting Agents

237 views

Determining the Role of Neutrophil-Like Cells in Lymphoma Cell Sensitivity to a Test Drug
4:23

Determining the Role of Neutrophil-Like Cells in Lymphoma Cell Sensitivity to a Test Drug

243 views

An Immunological Technique to Monitor Adjuvant-Mediated Cytotoxic T Lymphocyte Generation
4:45

An Immunological Technique to Monitor Adjuvant-Mediated Cytotoxic T Lymphocyte Generation

259 views

An Assay to Evaluate Immunodominance in Cytotoxic T Lymphocyte Responses
3:00

An Assay to Evaluate Immunodominance in Cytotoxic T Lymphocyte Responses

252 views

A Technique to Assess the Inhibitory Effect of Toxin Exposure on Miniature Excitatory Postsynaptic Currents (mEPSCs)
4:37

A Technique to Assess the Inhibitory Effect of Toxin Exposure on Miniature Excitatory Postsynaptic Currents (mEPSCs)

322 views

Measuring Macrophage Phagocytic Efficiency with Primaquine and Photodynamic Therapy
3:45

Measuring Macrophage Phagocytic Efficiency with Primaquine and Photodynamic Therapy

406 views

An Assay to Study the Impact of Photodynamic Treatment with a Photosensitizer on Macrophage Metabolic Activity
3:10

An Assay to Study the Impact of Photodynamic Treatment with a Photosensitizer on Macrophage Metabolic Activity

333 views

The Effect of Aspirin and Ibuprofen on the Phagocytic Ability of Macrophages
3:06

The Effect of Aspirin and Ibuprofen on the Phagocytic Ability of Macrophages

454 views

Isolation and Digestion of Neutrophil Proteins
4:50

Isolation and Digestion of Neutrophil Proteins

509 views

A STAGE Tip Procedure for Desalting and Purification of Peptides
5:43

A STAGE Tip Procedure for Desalting and Purification of Peptides

1.2K views

Imaging and Visualization Techniques

30 Videos

132 Minutes

Calcein Acetoxymethyl Staining to Evaluate Natural Killer Cell-Mediated Cytotoxicity toward Cancer Cells
2:53

Calcein Acetoxymethyl Staining to Evaluate Natural Killer Cell-Mediated Cytotoxicity toward Cancer Cells

460 views

In Vitro Stimulation and Visualization of Macrophage Extracellular Traps
4:31

In Vitro Stimulation and Visualization of Macrophage Extracellular Traps

371 views

Reporter Enzyme Fluorescence Imaging for In Vivo Tracking of Bacterial Infection
5:26

Reporter Enzyme Fluorescence Imaging for In Vivo Tracking of Bacterial Infection

303 views

Assessment of <em>Leishmania Virulence</em> within Cultured Macrophages
4:46

Assessment of Leishmania Virulence within Cultured Macrophages

328 views

Assessment of Intracellular Growth of <em>Coxiella</em> Mutants within Eukaryotic Cells
5:13

Assessment of Intracellular Growth of Coxiella Mutants within Eukaryotic Cells

251 views

Visualization of Internalized Anaerobic Bacteria within Host Endothelial Cells
4:19

Visualization of Internalized Anaerobic Bacteria within Host Endothelial Cells

284 views

A Technique to Assess the In Vivo Localization of Tumor-Specific Antibodies
4:13

A Technique to Assess the In Vivo Localization of Tumor-Specific Antibodies

292 views

Optical Coherence Tomography Imaging for Assessing Uveitis in a Mouse Model
3:12

Optical Coherence Tomography Imaging for Assessing Uveitis in a Mouse Model

360 views

Visualization of Bacteria in Bladder Biopsy Sections via Fluorescence In Situ Hybridization
6:10

Visualization of Bacteria in Bladder Biopsy Sections via Fluorescence In Situ Hybridization

326 views

Photoconversion-Based Leukocyte Tracking in Bacterial-Infected Transgenic Zebrafish
4:02

Photoconversion-Based Leukocyte Tracking in Bacterial-Infected Transgenic Zebrafish

295 views

Three-Dimensional Quantitative Phase Imaging for Characterizing Lymphocyte Subtypes
4:37

Three-Dimensional Quantitative Phase Imaging for Characterizing Lymphocyte Subtypes

340 views

Super-Resolution Imaging of NK Cell Immunological Synapse Formation on a Supported Lipid Bilayer
7:53

Super-Resolution Imaging of NK Cell Immunological Synapse Formation on a Supported Lipid Bilayer

305 views

An In Vitro Technique to Visualize Exocytosis of Secretory Granules in Mast Cells
3:34

An In Vitro Technique to Visualize Exocytosis of Secretory Granules in Mast Cells

318 views

A zWEDGI Technique to Visualize Fungal Pathogen-Infected Zebrafish Larvae
2:58

A zWEDGI Technique to Visualize Fungal Pathogen-Infected Zebrafish Larvae

275 views

The Photoconversion Technique for Exploring Inflammatory Cell Dynamics in Insect Pupae
3:44

The Photoconversion Technique for Exploring Inflammatory Cell Dynamics in Insect Pupae

333 views

Assessing Immunological Synapse Topology through Live-Cell Imaging
5:03

Assessing Immunological Synapse Topology through Live-Cell Imaging

262 views

An In Vivo Immunofluorescence Localization Method to Study Antibody Biodistribution
6:15

An In Vivo Immunofluorescence Localization Method to Study Antibody Biodistribution

345 views

A Luciferase-Fluorescent Reporter Influenza Virus to Track Viral Infections
3:49

A Luciferase-Fluorescent Reporter Influenza Virus to Track Viral Infections

323 views

Using Immuno-RNA Fluorescence In Situ Hybridization to Visualize SARS-CoV-2
2:59

Using Immuno-RNA Fluorescence In Situ Hybridization to Visualize SARS-CoV-2

398 views

Localized Surface Plasmon Resonance Imaging to Detect Protein Secretions from a Single Cell
5:00

Localized Surface Plasmon Resonance Imaging to Detect Protein Secretions from a Single Cell

327 views

Analyzing the Antigenic Relationship between Viruses using an Image-Based Microneutralization Assay
3:17

Analyzing the Antigenic Relationship between Viruses using an Image-Based Microneutralization Assay

221 views

An In Vivo Assay to Quantify Bacterial Phagocytosis in Adult <em>Drosophila</em> Flies
4:50

An In Vivo Assay to Quantify Bacterial Phagocytosis in Adult Drosophila Flies

284 views

Using DNA-Based Tension Probes to Assess Receptor Forces Applied by Immune Cells
5:58

Using DNA-Based Tension Probes to Assess Receptor Forces Applied by Immune Cells

219 views

Colorimetric Detection of a Bacterial Biomarker Using a Modified Litmus Test
3:04

Colorimetric Detection of a Bacterial Biomarker Using a Modified Litmus Test

294 views

A Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-Infected Cells
4:07

A Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-Infected Cells

367 views

Quantitative Time-Lapse Microscopy for Assessing Extracellular Matrix Stiffness-Dependent Bacterial Dissemination
4:24

Quantitative Time-Lapse Microscopy for Assessing Extracellular Matrix Stiffness-Dependent Bacterial Dissemination

235 views

TIRF Microscopy-Based Visualization of Phagosome Formation and Closure
4:13

TIRF Microscopy-Based Visualization of Phagosome Formation and Closure

298 views

Real-Time Analysis of Resident T-cell Migration in Different Tumor Regions Using Confocal Microscopy
4:23

Real-Time Analysis of Resident T-cell Migration in Different Tumor Regions Using Confocal Microscopy

238 views

An Immunofluorescence Method to Measure the Neutralization of Human Cytomegalovirus
4:05

An Immunofluorescence Method to Measure the Neutralization of Human Cytomegalovirus

376 views

Using <sup>23</sup>Na Magnetic Resonance Imaging to Measure Sodium in Muscle Tissues
3:16

Using 23Na Magnetic Resonance Imaging to Measure Sodium in Muscle Tissues

851 views

In Vitro and In Vivo Models

19 Videos

77 Minutes

Establishing an Infection Model Through Microinjection of Gastric Organoids with Pathogenic Bacteria
3:30

Establishing an Infection Model Through Microinjection of Gastric Organoids with Pathogenic Bacteria

362 views

<em>Candida albicans</em> Biofilm Formation in an In Vivo Mouse Model
3:50

Candida albicans Biofilm Formation in an In Vivo Mouse Model

402 views

Induction of Necrotizing Enterocolitis in Human Epithelial Enteroid Model
5:31

Induction of Necrotizing Enterocolitis in Human Epithelial Enteroid Model

200 views

A Mouse Tail Snip Procedure for Peripheral Blood Collection and Plasma Isolation
2:51

A Mouse Tail Snip Procedure for Peripheral Blood Collection and Plasma Isolation

2.3K views

An In Vitro Technique for UV Irradiation-Mediated Generation of Apoptotic T Cells
3:54

An In Vitro Technique for UV Irradiation-Mediated Generation of Apoptotic T Cells

1K views

Agarose-Based Preparation of Human Precision-Cut Lung Slices
4:26

Agarose-Based Preparation of Human Precision-Cut Lung Slices

763 views

An Assay to Measure Chemically-Induced Cytotoxicity in Human Precision-Cut Lung Slices
3:10

An Assay to Measure Chemically-Induced Cytotoxicity in Human Precision-Cut Lung Slices

355 views

Bacterial Culture Filtrate Protein Concentration using an Ultrafiltration Device
2:49

Bacterial Culture Filtrate Protein Concentration using an Ultrafiltration Device

397 views

A Size Exclusion Chromatography Technique to Purify Mycobacterial Extracellular Vesicles
4:38

A Size Exclusion Chromatography Technique to Purify Mycobacterial Extracellular Vesicles

366 views

Measuring Immune-Induced Inhibition of Seedling Growth
2:29

Measuring Immune-Induced Inhibition of Seedling Growth

292 views

An Assay to Evaluate Drug Efficacy against a <em>Mycobacterium tuberculosis</em> Infection Model
5:14

An Assay to Evaluate Drug Efficacy against a Mycobacterium tuberculosis Infection Model

314 views

A Focus-Forming Assay for Quantifying Virus Titers in the Organs of an Infected Mouse
6:23

A Focus-Forming Assay for Quantifying Virus Titers in the Organs of an Infected Mouse

514 views

Assessing the Antimicrobial Potential of Bacteriocin-Producing Murine Fecal Bacterial Isolates
2:49

Assessing the Antimicrobial Potential of Bacteriocin-Producing Murine Fecal Bacterial Isolates

291 views

A Riboflavin and UV Light Treatment of Platelet Products to Eliminate Bacteria
5:09

A Riboflavin and UV Light Treatment of Platelet Products to Eliminate Bacteria

220 views

In Vitro Measurement of α-Galactosidase A and Acid α-Glucosidase Enzyme Activity
3:20

In Vitro Measurement of α-Galactosidase A and Acid α-Glucosidase Enzyme Activity

341 views

An In Vitro Broth Microdilution Assay for the Screening of Antifungal Compounds
3:48

An In Vitro Broth Microdilution Assay for the Screening of Antifungal Compounds

424 views

An In Vitro Assay for Evaluating the Immunomodulatory Impact of Monocytes on Leukocyte Proliferation
2:46

An In Vitro Assay for Evaluating the Immunomodulatory Impact of Monocytes on Leukocyte Proliferation

242 views

An In Vitro Flow Investigation of the Effect of Stromal Cells on Leukocyte Recruitment
5:52

An In Vitro Flow Investigation of the Effect of Stromal Cells on Leukocyte Recruitment

268 views

A Method for Sample Preparation to Visualize Biofilm Forming Bacteria on Fungal Hyphae
4:37

A Method for Sample Preparation to Visualize Biofilm Forming Bacteria on Fungal Hyphae

331 views

Specialized Assays

67 Videos

280 Minutes

Real-Time Imaging of Macrophage Phagocytosis of IgG-Opsonized Red Blood Cells
4:33

Real-Time Imaging of Macrophage Phagocytosis of IgG-Opsonized Red Blood Cells

756 views

Measurement of Natural Killer Cell Migration in Response to Chemotactic Stimuli
2:29

Measurement of Natural Killer Cell Migration in Response to Chemotactic Stimuli

420 views

A Serum Bactericidal Assay for the Complement-Mediated Bactericidal Activity of Antibodies
5:40

A Serum Bactericidal Assay for the Complement-Mediated Bactericidal Activity of Antibodies

889 views

The Hemagglutination Inhibition Assay to Detect Serum Antibodies Against a Target Antigen
3:59

The Hemagglutination Inhibition Assay to Detect Serum Antibodies Against a Target Antigen

1.7K views

An Assay for Bacterial Extracellular Protein-Mediated Cytotoxicity on PMNs
3:14

An Assay for Bacterial Extracellular Protein-Mediated Cytotoxicity on PMNs

224 views

Cytotoxicity Assay of <em>Staphylococcus aureus</em> Strains on Neutrophils following Phagocytosis
3:35

Cytotoxicity Assay of Staphylococcus aureus Strains on Neutrophils following Phagocytosis

290 views

A Bacterial Infection Assay for Studying Anaerobic Bacteria-Host Cell Interactions
5:28

A Bacterial Infection Assay for Studying Anaerobic Bacteria-Host Cell Interactions

654 views

Assessment of Opsonophagocytic Killing Activity against Bacterial Pathogens
4:29

Assessment of Opsonophagocytic Killing Activity against Bacterial Pathogens

408 views

Assessing Internalized <em>Porphyromonas gingivalis</em> in Host Endothelial Cells
2:37

Assessing Internalized Porphyromonas gingivalis in Host Endothelial Cells

273 views

An In Vitro Assay to Study the Cytotoxic Capability of Cytokine-Induced Killer Cells
5:27

An In Vitro Assay to Study the Cytotoxic Capability of Cytokine-Induced Killer Cells

294 views

In Vitro Adhesion Assay to Detect the Adherence of Cancer Cells to Neutrophil Extracellular Traps
4:25

In Vitro Adhesion Assay to Detect the Adherence of Cancer Cells to Neutrophil Extracellular Traps

393 views

Evaluating Cytotoxicity and Membrane Integrity in Lung Slices via Lactate Dehydrogenase Assay
2:58

Evaluating Cytotoxicity and Membrane Integrity in Lung Slices via Lactate Dehydrogenase Assay

317 views

A Fluorescence-Based Assay Using Engineered Lymphocytes for Screening Immunomodulatory Compounds
2:57

A Fluorescence-Based Assay Using Engineered Lymphocytes for Screening Immunomodulatory Compounds

258 views

Monitoring B Cell Activation with Antigenic Liposomes using a Calcium-Flux Assay
3:54

Monitoring B Cell Activation with Antigenic Liposomes using a Calcium-Flux Assay

300 views

A Technique to Quantify Fungal Infection in Zebrafish Larvae
2:24

A Technique to Quantify Fungal Infection in Zebrafish Larvae

313 views

An Opsono-Adherence Assay for Assessing the Opsonization of Encapsulated Bacteria by Anti-Capsule Antibodies
6:34

An Opsono-Adherence Assay for Assessing the Opsonization of Encapsulated Bacteria by Anti-Capsule Antibodies

242 views

A Bead-Based Multiplex Immunoassay to Identify Pathogen-Specific Antibodies in Saliva
3:45

A Bead-Based Multiplex Immunoassay to Identify Pathogen-Specific Antibodies in Saliva

274 views

Quantitative Flow Cytometric Measurement of Fungal Spore Phagocytosis by Human Phagocytes
5:28

Quantitative Flow Cytometric Measurement of Fungal Spore Phagocytosis by Human Phagocytes

247 views

An Immunofluorescence Assay to Characterize and Quantify Mammalian Stress Granules
4:34

An Immunofluorescence Assay to Characterize and Quantify Mammalian Stress Granules

299 views

An Assay to Assess Phagocytosis and Oxidative Burst Activity In Granulocytes and Monocytes
4:59

An Assay to Assess Phagocytosis and Oxidative Burst Activity In Granulocytes and Monocytes

266 views

Quantification of Immune Mediator Levels in Human Mucosal Lining Fluid
2:55

Quantification of Immune Mediator Levels in Human Mucosal Lining Fluid

197 views

A Fluorogenic Peptide Cleavage Assay to Screen the Proteolytic Activity of Proteases
2:42

A Fluorogenic Peptide Cleavage Assay to Screen the Proteolytic Activity of Proteases

285 views

An Assay for Quantifying Antibody-Mediated Phagocytosis of Parasite-Infected Erythrocytes
6:56

An Assay for Quantifying Antibody-Mediated Phagocytosis of Parasite-Infected Erythrocytes

285 views

An In Vitro Assay for Measuring Neutrophil Serine Protease Activity Using a Fluorescent Reporter
2:59

An In Vitro Assay for Measuring Neutrophil Serine Protease Activity Using a Fluorescent Reporter

260 views

An In Vitro Assay for Studying the Cytotoxicity of Pre-Activated CD8<sup>+</sup> T Cells against Cancer Cells
5:04

An In Vitro Assay for Studying the Cytotoxicity of Pre-Activated CD8+ T Cells against Cancer Cells

470 views

A Microneutralization Assay to Measure Neutralizing Antibody Titers in Human Serum
5:33

A Microneutralization Assay to Measure Neutralizing Antibody Titers in Human Serum

461 views

An Assay to Detect Oxidative Burst in <em>Arabidopsis</em> Leaf Discs upon Immune Elicitation
2:58

An Assay to Detect Oxidative Burst in Arabidopsis Leaf Discs upon Immune Elicitation

550 views

An Assay for Detecting Reactive Oxygen Species Production in Rice Tissues upon Immune Elicitation
3:42

An Assay for Detecting Reactive Oxygen Species Production in Rice Tissues upon Immune Elicitation

355 views

An Assay for Studying Fcγ Receptor-Driven Phagocytosis in Monocyte Monolayers
3:30

An Assay for Studying Fcγ Receptor-Driven Phagocytosis in Monocyte Monolayers

235 views

A Charcoal Agar Resazurin Assay to Evaluate Test Compound Activity Against Mycobacteria
6:08

A Charcoal Agar Resazurin Assay to Evaluate Test Compound Activity Against Mycobacteria

261 views

An Assay for TLR-Dependent NF-&kcy;B/AP-1 Transcription Factor Signaling in Macrophages
4:08

An Assay for TLR-Dependent NF-кB/AP-1 Transcription Factor Signaling in Macrophages

240 views

A Real-Time In Vitro Cytolysis Assay to Evaluate the Potency of CAR T Cells
3:11

A Real-Time In Vitro Cytolysis Assay to Evaluate the Potency of CAR T Cells

364 views

An In Vitro Method to Determine the Anti-Apoptotic Activity of Antibodies against TNFα
5:54

An In Vitro Method to Determine the Anti-Apoptotic Activity of Antibodies against TNFα

245 views

A Vaccine-Pulsed Dendritic Cell-Based In Vitro Assay to Induce Lymphocyte Proliferation
5:16

A Vaccine-Pulsed Dendritic Cell-Based In Vitro Assay to Induce Lymphocyte Proliferation

299 views

A Hemocyte Disturbance Assay to Assess Hemocyte Re-Adhesion to Hematopoietic Pockets
2:19

A Hemocyte Disturbance Assay to Assess Hemocyte Re-Adhesion to Hematopoietic Pockets

239 views

An In Vitro Assay to Examine the Antibody-Dependent Enhancement of Viral Infection
5:35

An In Vitro Assay to Examine the Antibody-Dependent Enhancement of Viral Infection

256 views

An In Vitro Assay to Quantify the Intracellular Growth of Parasite in Macrophages
3:29

An In Vitro Assay to Quantify the Intracellular Growth of Parasite in Macrophages

267 views

A Dye Release Assay to Quantify Enzymatic Activity of Antimicrobial Proteins
6:01

A Dye Release Assay to Quantify Enzymatic Activity of Antimicrobial Proteins

362 views

A Spot Plate Assay for Evaluating Oxidative Stress Responses in Bacterial Strains
5:10

A Spot Plate Assay for Evaluating Oxidative Stress Responses in Bacterial Strains

627 views

Evaluating the Effectiveness of a Test Compound against Mycobacteria in Broth
2:20

Evaluating the Effectiveness of a Test Compound against Mycobacteria in Broth

235 views

A Peg Plate Biofilm Assay for Screening Antibacterial Agents
6:41

A Peg Plate Biofilm Assay for Screening Antibacterial Agents

500 views

A Barcoding Assay for Phenotypic and Functional Characterization of Immune Cells
5:42

A Barcoding Assay for Phenotypic and Functional Characterization of Immune Cells

225 views

A CFSE-Based Assay to Monitor Skin Dendritic Cell Migration to Draining Lymph Nodes in Infected Mice
4:34

A CFSE-Based Assay to Monitor Skin Dendritic Cell Migration to Draining Lymph Nodes in Infected Mice

287 views

Utilizing Bead-Supported Lipid Bilayers to Investigate the Synaptic Output from T Cells
3:49

Utilizing Bead-Supported Lipid Bilayers to Investigate the Synaptic Output from T Cells

237 views

A Deferred Growth Inhibition Assay to Evaluate the Effect of Bacteria-Derived Antimicrobial Compounds
3:37

A Deferred Growth Inhibition Assay to Evaluate the Effect of Bacteria-Derived Antimicrobial Compounds

406 views

A Turbidimetric Analysis to Monitor Collagen-Induced Aggregation of Pretreated Human Platelets
5:42

A Turbidimetric Analysis to Monitor Collagen-Induced Aggregation of Pretreated Human Platelets

303 views

Assessing the Effectiveness of an Antiviral Test Compound through a Viral Inactivation Assay
3:41

Assessing the Effectiveness of an Antiviral Test Compound through a Viral Inactivation Assay

257 views

A Viral Attachment Assay for Screening of Antiviral Compounds
2:47

A Viral Attachment Assay for Screening of Antiviral Compounds

600 views

An Assay to Evaluate the Effect of Test Compounds on Viral Entry and Fusion in Host Cells
2:38

An Assay to Evaluate the Effect of Test Compounds on Viral Entry and Fusion in Host Cells

268 views

A Molecular-Based Assay for Quantifying Influenza A and B Viruses
3:49

A Molecular-Based Assay for Quantifying Influenza A and B Viruses

266 views

A Microtiter Plate Method to Assess the Minimum Inhibitory Concentration of an Antibiotic
5:16

A Microtiter Plate Method to Assess the Minimum Inhibitory Concentration of an Antibiotic

1K views

An Assay to Study the Impact of Extracellular Matrix Stiffness on Bacterial Infection
4:10

An Assay to Study the Impact of Extracellular Matrix Stiffness on Bacterial Infection

281 views

Determining Antiviral Efficacy of Experimental Drugs Through a Plaque Assay
2:48

Determining Antiviral Efficacy of Experimental Drugs Through a Plaque Assay

624 views

An Assay to Determine Polymicrobial Biofilm Initiation on Virus-Infected Cells
3:57

An Assay to Determine Polymicrobial Biofilm Initiation on Virus-Infected Cells

254 views

An ATP Utilization Assay for Determining Bacterial Survival Under Antibiotics Treatment
2:56

An ATP Utilization Assay for Determining Bacterial Survival Under Antibiotics Treatment

298 views

Flow Cytometry-Based Quantification of Therapy-Induced Senescent Cancer Cells
4:19

Flow Cytometry-Based Quantification of Therapy-Induced Senescent Cancer Cells

357 views

Detecting Bacterial Contamination Using Magneto-fluorescent Nanosensors
3:08

Detecting Bacterial Contamination Using Magneto-fluorescent Nanosensors

255 views

A Solid Phase Adherence Assay to Assess Biofilm Formation
4:07

A Solid Phase Adherence Assay to Assess Biofilm Formation

462 views

An Assay to Study Bile-Salt Induced Biofilm Formation through EPS Matrix Detection
2:50

An Assay to Study Bile-Salt Induced Biofilm Formation through EPS Matrix Detection

285 views

Detection of Biofilm Disassembly Through a Dispersion Assay
2:49

Detection of Biofilm Disassembly Through a Dispersion Assay

355 views

An Assay for Real-Time Monitoring of Extracellular pH in Dental Biofilms
6:52

An Assay for Real-Time Monitoring of Extracellular pH in Dental Biofilms

213 views

A FRET Flow Cytometry Technique to Detect Tau-Seed Induced Reporter Protein Aggregation
4:11

A FRET Flow Cytometry Technique to Detect Tau-Seed Induced Reporter Protein Aggregation

396 views

Multiplexed Quantitative Bacterial Detection using Dye-Labeled Magnetic Microspheres
3:54

Multiplexed Quantitative Bacterial Detection using Dye-Labeled Magnetic Microspheres

386 views

Assessing Functional SARS-CoV-2 Neutralizing Antibodies
5:30

Assessing Functional SARS-CoV-2 Neutralizing Antibodies

884 views

A Degranulation Assay to Monitor Neutrophil Activation and Gelatinase Release
3:50

A Degranulation Assay to Monitor Neutrophil Activation and Gelatinase Release

739 views

An Assay to Monitor Degranulation of Azurophilic Granules in Neutrophils following Activation
3:05

An Assay to Monitor Degranulation of Azurophilic Granules in Neutrophils following Activation

476 views

Assays for Qualitative and Quantitative Assessment of Mouse Urine Glucose Concentrations
4:42

Assays for Qualitative and Quantitative Assessment of Mouse Urine Glucose Concentrations

784 views

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