علم الوراثة ، العدد 181 ، الاستجابة لتلف الحمض النووي ، DSB ، إصلاح DSB ، DSB المستحث CAS9 ، تسلسل الجيل التالي ، علم الجينوم Protocols and Video Articles

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Analysis of DNA Double-strand Break (DSB) Repair in Mammalian Cells

Authors

Author

Andrei Seluanov¹, Zhiyong Mao¹, Vera Gorbunova¹

Institution

¹Department of Biology, University of Rochester

Journal (Biology)

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

Authors

Author

Shenglan Li*^1,2, Haipeng Xue*^1,2, Bo Long^1,2,5, Li Sun^1,2,6, Tai Truong^1,2,4,7, Ying Liu^1,2,3

Institution

¹Department of Neurosurgery, The University of Texas Health Science Center at Houston, ²Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, ³The Senator Lloyd & B. A. Bentsen Center for Stroke Research, Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, ⁴Summer Research Program, Office of Educational Programs, The University of Texas Health Science Center at Houston, ⁵Department of Anesthesiology, Shengjing Hospital, China Medical University, ⁶Department of Oncology, Renji Hospital, Shanghai Jiaotong University School of Medicine, ⁷Biology Department, University of West Georgia

Journal (Developmental Biology)

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Authors

Author

Daniel E. Bauer*^1,2,3, Matthew C. Canver*¹, Stuart H. Orkin^1,2,3,4

Institution

¹Harvard Medical School, ²Division of Hematology/Oncology, Boston Children's Hospital, ³Department of Pediatric Oncology, Dana-Farber Cancer Institute, ⁴Howard Hughes Medical Institute

Journal (Biology)

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Authors

Author

Linyuan Ma*¹, Lydia Jang*¹, Jian Chen², Jun Song¹, Dongshan Yang¹, Jifeng Zhang¹, Y. Eugene Chen¹, Jie Xu¹

Institution

¹Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical Center, ²Celetrix LLC

Journal (Genetics)

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Authors

Author

Qiang Yan*¹, Yingying He*^2,3, Yang Yue^2,3, Luyao Jie¹, Tingmei Wen^2,3, Yumiao Zhao^2,3, Min Zhang², Tingting Zhang²

Institution

¹State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, ²Research Institute of Applied Biology, Shanxi University, ³School of Life Science, Shanxi University

Journal (Biology)

| Methods Collections

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Authors

Author

Changkun Hu*¹, Tyler Doerksen*², Taylor Bugbee¹, Nicholas A. Wallace¹, Rachel Palinski^2,3

Institution

¹Division of Biology, Kansas State University, ²Kansas State Veterinary Diagnostic Laboratory, Kansas State University, ³Department of Diagnostic Medicine/Pathobiology, Kansas State University

Journal (Genetics)

| Methods Collections

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