Shanxi University 1 article published in JoVE Biology Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology Qiang Yan*1, Yingying He*2,3, Yang Yue2,3, Luyao Jie1, Tingmei Wen2,3, Yumiao Zhao2,3, Min Zhang2, Tingting Zhang2 1State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 2Research Institute of Applied Biology, Shanxi University, 3School of Life Science, Shanxi University This study provides a systematically optimized procedure of CRISPR/Cas9 ribonuclease-based construction of homozygous locust mutants as well as a detailed method for cryopreservation and resuscitation of the locust eggs.
