Generación eficiente de hiPSC Neuronales Lineage Reporteros Knockin concreto utilizando la CRISPR / Cas9 y Cas9 Doble Sistema Nickase

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Citado 5 veces

14:46 min

28 de mayo de 2015

10.3791/52539-v

28 de mayo de 2015

10.5K vistas

Shenglan Li^*¹^,² , Haipeng Xue^*¹^,² , Bo Long¹^,²^,⁵ , Li Sun¹^,²^,⁶ , Tai Truong¹^,²^,⁴^,⁷ , Ying Liu¹^,²^,³

¹Department of Neurosurgery, The University of Texas Health Science Center at Houston, ²Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, ³The Senator Lloyd & B. A. Bentsen Center for Stroke Research, Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, ⁴Summer Research Program, Office of Educational Programs, The University of Texas Health Science Center at Houston, ⁵Department of Anesthesiology, Shengjing Hospital, China Medical University, ⁶Department of Oncology, Renji Hospital, Shanghai Jiaotong University School of Medicine, ⁷Biology Department, University of West Georgia

^* These authors contributed equally

Las herramientas de edición del genoma, como el sistema CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas (CRISPR-associated), han mejorado en gran medida la eficiencia de la selección de genes en células madre pluripotentes inducidas humanas (hiPSC). Este manuscrito describe un protocolo para generar un indicador de hiPSC específico del linaje utilizando la recombinación homóloga asistida por el sistema CRISPR/Cas.