癌症研究，第193期，CRISPR/Cas9，基因组工程，白血病发生，造血干细胞，AAV，突变，同源重组，功能获得突变 Protocols and Video Articles

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CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus (AAV) and 2-Cell Embryo Electroporation

Authors

Author

Daniel J. Davis^1,2,4, James F. McNew², Jennifer N. Walls³, Christine E. Bethune³, Payton S. Oswalt³, Elizabeth C. Bryda^1,2,3,4

Institution

¹Animal Modeling Core, University of Missouri, ²Comparative Medicine Program, University of Missouri, ³Rat Resource and Research Center, University of Missouri, ⁴Department of Veterinary Pathobiology, University of Missouri

Journal (Genetics)

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Authors

Author

Kyle E. Simpson¹, Shoaib Faizi¹, Ravichandra Venkateshappa¹, Mandy Yip¹, Raj Johal¹, Damon Poburko¹, Yen May Cheng¹, Diana Hunter¹, Eric Lin¹, Glen F. Tibbits¹, Thomas W. Claydon¹

Institution

¹Department of Biomedical Physiology and Kinesiology, Simon Fraser University

Journal (Biology)

| Methods Collections

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Authors

Author

Kanut Laoharawee^1,2,3, Matthew J. Johnson^1,2,3, Walker S. Lahr^1,2,3, Joseph J. Peterson^1,2,3, Beau R. Webber^1,2,3, Branden S. Moriarity^1,2,3

Institution

¹Department of Pediatrics, University of Minnesota, ²Center for Genomic Engineering, University of Minnesota, ³Masonic Cancer Center, University of Minnesota

Journal (Biology)

| Methods Collections

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Authors

Author

Daniel E. Bauer*^1,2,3, Matthew C. Canver*¹, Stuart H. Orkin^1,2,3,4

Institution

¹Harvard Medical School, ²Division of Hematology/Oncology, Boston Children's Hospital, ³Department of Pediatric Oncology, Dana-Farber Cancer Institute, ⁴Howard Hughes Medical Institute

Journal (Biology)

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Authors

Author

Qiang Yan*¹, Yingying He*^2,3, Yang Yue^2,3, Luyao Jie¹, Tingmei Wen^2,3, Yumiao Zhao^2,3, Min Zhang², Tingting Zhang²

Institution

¹State Key Laboratory of Cotton Biology, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, ²Research Institute of Applied Biology, Shanxi University, ³School of Life Science, Shanxi University

Journal (Biology)

| Methods Collections

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Authors

Author

Na Tang^1,2, Yaoyao Zhang¹, Miriam Pedrera¹, Pengxiang Chang¹, Susan Baigent¹, Katy Moffat¹, Zhiqiang Shen², Venugopal Nair¹, Yongxiu Yao¹

Institution

¹The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, ²Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

Journal (Immunology and Infection)

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Education

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CRISPR

JoVE Core (Core: Biología Molecular)

CRISPR

JoVE Core (Core: Biología)

CRISPR and crRNAs

JoVE Core (Core: Biología Molecular)

Bacterial Transformation- Concept

JoVE Lab (Lab Manual: Biología)

What is Genetic Engineering?

JoVE Core (Core: Biología)

Leveling Effect and Non-Aqueous Acid-Base Solutions

JoVE Core (Core: Organic Chemistry)

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