Gingival Sulcus
Gingival Sulcus - Related Videos
09:47
Isolation, Processing and Analysis of Murine Gingival Cells
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Cited by 56 •
2013
This study describes an efficient technique to isolate and process gingival tissues from the mouse oral cavity in order to produce a single-cell culture. The resulting cells can be further used for flow cytometry analysis and molecular studies.
03:19
Isolation of Gingival Immune Cell Network from Murine Teeth Blocks
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2025
This video demonstrates the isolation procedure of the gingival immune cell network from a mouse model. The enzymatic digestion releases the gingival tissue from the attached teeth and bone, which is further mechanically digested, releasing immune cells from the gingiva. The isolated cells are ready for immunological experiments.
05:07
Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network
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Cited by 30 •
2016
We have established a technique for the isolation, phenotypic characterization and functional analysis of immune cells from murine gingiva.
04:46
In Situ Detection of Bacteria in Mouse Gingival Tissue Sections
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2026
Source: Choi, Y. S., et al. In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA. J. Vis. Exp. (2015).This video demonstrates the in situ detection of bacteria in mouse gingival tissue sections using digoxin-labeled DNA probes targeting bacterial DNA. The section is incubated with the probe. Alkaline phosphatase-conjugated antibodies specific to digoxin are added. Endogenous phosphatases are inactivated, and a colored precipitate is...
08:03
Visualization of the Superior Ocular Sulcus during Danio rerio Embryogenesis
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2019
Here, we present a standardized series of protocols to observe the superior ocular sulcus, a recently-identified, evolutionarily-conserved structure in the vertebrate eye. Using zebrafish larvae, we demonstrate techniques necessary to identify factors that contribute to the formation and closure of the superior ocular sulcus.
FAQs
The junctional epithelium forms an important part of the epithelial barrier at the tooth-tissue interface. By helping separate periodontal tissues from the external oral environment, it supports local protection. Its condition is therefore biologically significant: when bacterial plaque disrupts this barrier, the surrounding periodontal tissues can develop an inflammatory response.
Bacterial plaque can shift the sulcus from a protective, healthy state toward inflammation by disrupting the epithelial barrier. This change links the local microbial community with host tissue responses. Studying that transition helps explain how oral bacteria can affect periodontal tissues without treating the sulcus as an isolated anatomical space.
Gingival crevicular fluid contributes to defense by carrying immune cells, antibodies, and other molecules into the sulcus. These components create a local protective response at the boundary between oral tissues and the external environment. Changes in this fluid can therefore provide biologically relevant information about host defense and inflammation in periodontal tissues.
The gingival sulcus brings oral microbial communities into close relationship with epithelial tissues and local immune defenses. Its position at the external environment makes it useful for examining how bacterial plaque interacts with tissue barriers and immune components. This interface provides a focused setting for studying the biological balance between protection and inflammation.
Sulcus-depth probing provides a clinical measure of the space around a tooth and can be used to assess periodontal conditions. The measurement connects a visible or measurable clinical feature with the biology of the epithelial barrier and surrounding tissues. In research and practice, it helps relate local sulcus changes to periodontal health or inflammation.
Biologists examine the gingival sulcus to connect three processes: the composition of oral microbial communities, the condition of the epithelial barrier, and the host immune response. This integrated view helps clarify how bacterial plaque is associated with inflammation and periodontal disease. It also supports interpretation of clinical observations involving periodontal tissues.
Research on this site can provide information about local immune defense, antibody distribution, bacterial plaque effects, and inflammatory changes in periodontal tissues. Because the sulcus contains both tissue-associated barriers and fluid carrying immune components, it links cellular and molecular responses with the surrounding oral microbial environment. This makes it valuable in biology-focused periodontal research.