Lactobacillus
Lactobacillus - Related Videos
01:49
An Assay To Evaluate the Anticancer Effects of Lactobacillus Cell-Free Supernatant
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2025
This video demonstrates an in vitro assay to study the anticancer effects of Lactobacillus cell-free supernatant (LCFS). Upon exposing colorectal cancer spheroids to varying doses of LCFS, the interaction with the bacterial metabolites in LCFS induces apoptosis in the cancer cells, disrupting the spheroid structure and leading to cell death in a dose-dependent manner.
06:06
Adjunctive Diode Laser Therapy and Probiotic Lactobacillus Therapy in the Treatment of Periodontitis and Peri-Implant Disease
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Cited by 1 •
2022
This article describes two protocols: 1) adjunctive diode laser therapy for treating periodontitis and 2) probiotic Lactobacillus therapy for treating peri-implant disease, with emphasis on the laser usage mode (inside or outside pocket), laser application regimen (single or multiple sessions), and a probiotic protocol of professional and home administration.
11:09
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
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Cited by 5 •
2011
We describe a multiplex method for the detection of microorganisms within a sample using oligonucleotide-coupled fluorescent beads. Amplicon from all organisms within a sample is hybridized to a panel of probe-coupled beads. A Luminex or Bio-Plex instrument is used to query each bead for bead type and hybridization signal.
08:38
Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods
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Cited by 14 •
2016
A production protocol is described for the small-scale production of probiotic fermented dairy drink with the aid of a novel bacterial starter culture.
09:20
Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay
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Cited by 8 •
2014
In vitro adherence assays can be used to study the attachment of Streptococcus pneumoniae to epithelial cell monolayers and to investigate potential interventions such as the use of probiotics for inhibiting pneumococcal colonization.
FAQs
NAD+ regeneration allows carbohydrate breakdown to continue when fermentation converts metabolic products into lactic acid. By restoring this oxidized cofactor, the cells maintain the reactions needed for ongoing energy-yielding metabolism. This connection explains how carbohydrate availability can support acid production and influence the chemical conditions surrounding a Lactobacillus population.
Accumulated lactic acid lowers the pH of the surrounding environment, creating conditions that inhibit many competing microorganisms. The effect is both ecological and practical: Lactobacillus can alter which organisms remain active in a community, while the resulting acidification helps preserve fermented foods by discouraging susceptible competitors.
Their presence in the gastrointestinal and reproductive tracts makes Lactobacillus species useful for examining how microbial metabolism relates to host-associated communities. Researchers can investigate where these bacteria occur, how their acid-producing activity changes local conditions, and how those changes may shape interactions among microbes and their host environment.
In food production, carbohydrate fermentation by Lactobacillus generates lactic acid and progressively acidifies the food environment. That chemical shift can suppress many competing microorganisms, linking bacterial metabolism with preservation. Studying this process helps explain how fermentation changes food stability and why particular microbial activities matter during production.
Probiotic research examines Lactobacillus within the broader context of host-associated microbiota and microbial activity. Relevant questions include how these bacteria fit into gastrointestinal or reproductive-tract communities and how their fermentation metabolism changes local conditions. This approach distinguishes the study of microbial mechanisms and interactions from simply identifying bacterial presence.
Lactobacillus provides a model for examining how metabolism shapes microbial communities. Carbohydrate use, NAD+ regeneration, and lactic acid release connect cellular activity with environmental acidification, competition, and community composition. These relationships can be studied in fermented foods, host microbiota, and other biological settings where organisms influence one another through changed chemical conditions.