Single-probe Scms
Single-probe Scms - Related Videos
15:00
Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
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Cited by 44 •
2016
Here, we present protocols to perform both ambient mass spectrometry imaging (MSI) of tissues and in-situ live single cell MS (SCMS) analysis using the single-probe, which is a miniaturized multifunctional device for MS analysis.
10:53
Scanning-probe Single-electron Capacitance Spectroscopy
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2013
Scanning-probe single-electron capacitance spectroscopy facilitates the study of single-electron motion in localized subsurface regions. A sensitive charge-detection circuit is incorporated into a cryogenic scanning probe microscope to investigate small systems of dopant atoms beneath the surface of semiconductor samples.
07:26
Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
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Cited by 9 •
2016
PI(4,5)P2 regulates various cellular functions, but its nanoscale organization in the cell plasma membrane is poorly understood. By labeling PI(4,5)P2 with a dual-color fluorescent probe fused to the Pleckstrin Homology domain, we describe a novel approach to study the PI(4,5)P2 spatial distribution in the plasma membrane at the nanometer scale.
08:07
Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
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Cited by 1 •
2025
This protocol aims to preserve cell metabolites for single-cell mass spectrometry (SCMS) by combining volatile salt solution washing, rapid liquid nitrogen quenching, freeze-drying, and −8 °C storage. It demonstrates that liquid nitrogen quenching is essential for maintaining metabolite profiles, while prolonged cold storage should be minimized to prevent metabolic alterations.
11:00
Hand Controlled Manipulation of Single Molecules via a Scanning Probe Microscope with a 3D Virtual Reality Interface
0 Views •
Cited by 6 •
2016
We demonstrate the precise manipulation of individual organic molecules on a metal surface with the tip of a scanning probe microscope driven in 3D by the experimenter's hand using a motion capture system and fully immersive virtual reality goggles.
FAQs
The fine capillary probe first accesses a selected cell and withdraws a small volume. Solvent extraction helps release chemical constituents from that sample, after which electrospray ionization transfers the resulting analytes into the mass spectrometer. The instrument analyzes them according to mass-to-charge ratio, producing a molecular profile tied to the sampled cell.
Bulk measurements combine material from many cells, so differences among individual cells may be averaged into a single result. Sampling one selected cell preserves cell-to-cell variation in metabolites, lipids, drugs, or other analytes. This resolution allows chemical investigations to connect molecular profiles with particular cells rather than only with a mixed population.
Because the probe removes only a small volume, the approach can be applied to living cells while retaining access to their chemical constituents. Selecting the cell before sampling also links the measured chemistry to a defined cellular source. Together, these features support investigations of variation and response without relying exclusively on measurements from pooled cell material.
An experiment begins by choosing an individual cell and positioning a fine capillary probe to collect a small sample. The collected material undergoes solvent extraction, and electrospray ionization introduces the extracted analytes into a mass spectrometer. Mass-to-charge measurements then generate chemical information associated with that specific cell, rather than with a bulk sample.
It is suited to studies that ask how chemistry varies among cells or changes in response to a drug. The technique supports analysis of cellular metabolism, pharmacological response, and disease-related chemistry, while also measuring constituents such as metabolites and lipids. Its single-cell readout helps relate those chemical patterns to individual cellular sources.
By linking molecular profiles to individual cells, single-probe SCMS adds cellular context to chemical measurements. In chemistry-focused research, that linkage can show whether an observed metabolite, lipid, drug, or other analyte pattern is associated with a particular cell or reflects a broader population measurement. This supports biological, medical, and translational investigations of cellular chemistry.