Aseptic Technique Procedure
Aseptic Technique Procedure - Related Videos
10:48
Aseptic Technique in Environmental Science
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Luisa Ikner Aseptic technique is a fundamental skill widely practiced in the field of environmental microbiology that requires a balance of mindfulness and practice in the laboratory. Proper use of this technique reduces the likelihood of bacterial or fungal contamination of reagents, culture media, and environmental samples. Aseptic technique is also vital to ensure data integrity and...
18:00
Aseptic Laboratory Techniques: Plating Methods
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Cited by 438 •
2012
When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.
11:43
Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
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Cited by 24 •
2012
When working in a laboratory, it is imperative to minimize sources of contamination. Aseptic technique refers to procedures that permit transfer of cultures and reagents while avoiding contact with non-sterile surfaces. Serological pipettes and micropipettors are used to measure precise volumes without compromising sterility of solutions used in experiments.
09:46
Construction and Evaluation of a Murine Calvarial Osteolysis Model by Exposure to CoCrMo Particles in Aseptic Loosening
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Cited by 9 •
2018
This manuscript describes a murine calvarial osteolysis model by exposure to CoCrMo particles, which constitutes an ideal animal model for assessing the interactions between wear particles and various cells in aseptic loosening.
12:40
A Modified Murine Heterotopic Heart Transplant Protocol Matching Contemporary Standards of Aseptic Technique, Anesthesia, and Analgesia
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2022
The present paper describes a modified technique for heterotopic vascularized cardiac transplantation with updated aseptic technique, analgesia, and anesthesia.
FAQs
Microbial contamination can alter experimental conditions and interfere with the interpretation of cell, embryo, tissue, or organoid studies. When unwanted microorganisms enter a culture or sample, observed growth and differentiation may no longer reflect the intended experiment. Aseptic technique therefore protects biological material and strengthens conclusions about developmental behavior.
The procedure combines hand hygiene, protective clothing, disinfected surfaces, sterilized instruments, and controlled handling to reduce opportunities for contamination at different stages of an experiment. This layered approach is important because protecting the work area alone does not address contamination introduced through hands, equipment, or transfers. Consistency across these measures supports reproducible results.
Reliability depends on maintaining clean hands, appropriate protective clothing, disinfected work surfaces, sterilized instruments, and controlled manipulation of samples. Inconsistent attention to any of these conditions can increase contamination risk during transfers or other manipulations. Applying the same practices across experiments helps distinguish genuine developmental effects from changes caused by handling conditions.
A basic workflow begins with hand hygiene and suitable protective clothing, followed by preparation of a disinfected work surface and sterilized instruments. Researchers then handle or transfer the culture, embryo, tissue, or organoid under controlled conditions. Maintaining these practices throughout manipulation helps reduce the chance that unwanted microorganisms enter valuable biological samples.
Before manipulation, the work area should be disinfected, instruments should be sterilized, and appropriate protective clothing should be available. Hand hygiene also forms part of the preparation. These measures establish the conditions needed for controlled handling of cultures, embryos, tissues, or organoids and help protect samples from contamination during transfers and related experimental procedures.
Researchers apply aseptic technique when culturing or manipulating cells, embryos, tissues, and organoids. These systems are used to examine development, morphogenesis, and cell fate, so contamination could interfere with the growth or differentiation being measured. Protecting the samples helps ensure that experimental observations reflect the study conditions rather than unwanted microbial effects.