Kidney Regeneration
Kidney Regeneration - Related Videos
10:42
Normothermic Machine Perfusion of Rat Kidneys for Transplantation
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2026
This protocol describes the ex vivo normothermic machine perfusion of a rat kidney for subsequent transplantation into a recipient rat.
06:25
Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury
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Cited by 27 •
2015
Here we present a reliable method to study adult kidney regeneration by inducing acute kidney injury by gentamicin injection. We show that injury is dependent on gentamicin dosage and environmental temperature using in situ hybridization to label lhx1a+ developing new nephrons.
08:19
The Mouse Isolated Perfused Kidney Technique
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Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
01:45
Kidney Structure
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2019
The kidneys are two large bean-shaped organs located in the upper abdomen. They filter the blood several times a day to remove toxins and rebalance water and electrolytes of the circulatory system via the renal veins. The kidneys receive blood directly from the heart via the renal arteries. These arteries enter the kidney at the hilum, the concave surface of the bean, where they branch and divide into smaller vessels and capillaries. The renal cortex is the thick outer layer of the kidney. It...
07:27
Laser Ablation of the Zebrafish Pronephros to Study Renal Epithelial Regeneration
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Cited by 43 •
2011
Acute kidney injury (AKI) in humans is a common clinical problem caused by damage to the epithelial cells that comprise kidney nephrons, and AKI is associated with high mortality rates of 50-70%1. Following epithelial cell destruction, nephrons have a limited ability to regenerate, though the mechanisms and limitations that guide this phenomenon remain poorly understood. In this video article, we describe our technique for targeted laser ablation of kidney nephron cells in the zebrafish...
FAQs
After injury, surviving tubular epithelial cells can re-enter the cell cycle, proliferate, migrate, and redifferentiate. This sequence matters because repair requires more than cell multiplication: cells must also regain an appropriate differentiated state. In developmental biology, tracking these transitions helps researchers assess whether damaged tubules are moving toward functional recovery.
These pathways may influence repair because they also guide nephron development. Wnt signaling and GDNF-RET signaling therefore provide developmental references for interpreting how injured renal tissue responds. Studying them does not simply describe cell proliferation; it asks whether repair reactivates regulatory programs associated with nephron formation, helping connect injury recovery with the developmental biology of the kidney.
Researchers can distinguish effective regeneration from maladaptive scarring by examining whether the repair response supports restoration of renal tissue and function rather than ending in scar formation. This comparison is important because cellular activity alone does not establish successful regeneration. In developmental biology, the quality of the outcome matters as much as the occurrence of proliferation or migration.
In a developmental biology study, researchers can follow surviving tubular epithelial cells through cell-cycle re-entry, proliferation, migration, and redifferentiation. They can then relate these cellular changes to Wnt or GDNF-RET signaling and to the condition of renal tissue. This workflow connects observable repair behavior with developmental mechanisms and the distinction between regeneration and scarring.
Research on kidney regeneration supports tissue engineering by identifying cellular behaviors and signaling pathways that could guide restoration of damaged renal tissue. It also informs strategies intended to improve recovery from renal disease. These applications remain translational goals rather than solved outcomes, because current knowledge has not yet overcome the challenge of recreating fully functional nephrons.
The central challenge is recreating fully functional nephrons, even when research identifies cellular responses and developmental signaling associated with repair. This limitation shows why restoration of renal tissue cannot be judged only by the presence of proliferation or signaling activity. It keeps kidney regeneration a major developmental biology problem with direct relevance to organoids and tissue engineering.