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Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury
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Developmental Biology
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JoVE Journal Developmental Biology
Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury

Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury

DOI:
10.3791/51912-v

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06:25 min

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August 03, 2015

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, ,
1Nephrology Division, Department of Medicine,Massachusetts General Hospital, 2Basic Sciences Division,Fred Hutchinson Cancer Research Center, 3Department of Genetics,Harvard Medical School

Chapters

  • 00:05Title
  • 01:05Advanced Preparation for Injection
  • 01:58Intraperitoneal Injection of Gentamicin
  • 03:29Post Injection Observation of Injury and Care of Recovering Fish
  • 04:24Results: Kidney Regeneration by Gentamicin Induced Injury
  • 05:48Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a reliable method to study adult kidney regeneration by inducing acute kidney injury by gentamicin injection. We show that injury is dependent on gentamicin dosage and environmental temperature using in situ hybridization to label lhx1a+ developing new nephrons.

Tags

Kidney RegenerationAdult ZebrafishGentamicin-induced InjuryNephronNeonephrogenesisLhx1aNephron Progenitor CellsIntraperitoneal InjectionVisual ScreeningOptimal Gentamicin DosageKidney Regeneration At Higher Temperatures

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