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JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury
 

Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury

Article doi: 10.3791/51912
August 3rd, 2015 •
1, 1,2, 1,3
1Nephrology Division, Department of Medicine, Massachusetts General Hospital, 2Basic Sciences Division, Fred Hutchinson Cancer Research Center, 3Department of Genetics, Harvard Medical School

Summary
Automatic Translation
August 3rd, 2015

Here we present a reliable method to study adult kidney regeneration by inducing acute kidney injury by gentamicin injection. We show that injury is dependent on gentamicin dosage and environmental temperature using in situ hybridization to label lhx1a+ developing new nephrons.

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