Endothelial Monolayer
Endothelial Monolayer - Related Videos
03:39
Studying Lymphocyte Transmigration Across a Microvascular Endothelial Cell Monolayer
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2025
This video demonstrates an experimental investigation of lymphocyte transmigration across a blood-brain barrier (BBB) endothelium model. A monolayer of human brain microvascular endothelial cells (HBMECs) is generated in vitro, and peripheral blood mononuclear cells (PBMCs) containing lymphocytes are added to the monolayer. The cells are then incubated to allow lymphocyte transmigration, and the migrated cells are quantified using flow cytometry.
08:51
A Real-time Electrical Impedance Based Technique to Measure Invasion of Endothelial Cell Monolayer by Cancer Cells
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Cited by 68 •
2011
This article describes an in vitro technique for monitoring cancer cells invading through a monolayer of endothelial cells. The data is acquired in real-time as a function of changes in impedance on the surface of electrodes at the well bottom.
10:46
Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
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Cited by 25 •
2013
Intercellular Ca2+-waves are driven by gap junction channels and hemichannels. Here, we describe a method to measure intercellular Ca2+-waves in cell monolayers in response to a local single-cell mechanical stimulus and its application to investigate the properties and regulation of gap junction channels and hemichannels.
12:43
On-Chip Endothelial Inflammatory Phenotyping
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Cited by 3 •
2012
Microfluidic flow chambers etched by photolithography and fabricated from PDMS are applied to probe functional outcomes associated with EC dysfunction and inflammation. In a representative experiment, the ability of differential shear stress to modulate monocytic cell adhesion to cytokine activated EC monolayers is demonstrated.
10:24
Quantitation of Endothelial Cell Adhesiveness In Vitro
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Cited by 10 •
2015
We report an in vitro method that allows the quantitation of the actual number of adhesive cells within an endothelial cell monolayer.
FAQs
Cell-cell junctions help maintain continuity across the endothelial layer and regulate how readily substances move between adjacent cells. Their condition therefore influences whether the interface remains selectively permeable or becomes disrupted. Examining junctional changes can help researchers connect structural alterations with increased vascular permeability during inflammation or infection-related tissue injury.
The cytoskeleton supports the organization and function of endothelial cell junctions, so changes in this internal framework can affect barrier stability and transport. It provides a mechanistic link between cellular structural responses and altered permeability. In infection studies, this relationship helps explain how inflammatory conditions may convert a regulated vascular interface into a more permissive one.
Inflammatory cytokines can modify endothelial properties in ways that promote leukocyte adhesion to the cell layer and subsequent transmigration across it. This response links chemical inflammatory signals with physical immune-cell trafficking. Measuring these changes helps clarify how circulating leukocytes leave the bloodstream and enter tissues during vascular inflammation associated with infection.
Selective permeability indicates that the endothelial interface does not simply block or permit all movement; it regulates transport between circulation and tissues. Changes in this property can signal barrier disruption and altered vascular behavior. Studying permeability therefore provides a way to evaluate how inflammatory signals, microbial products, or infection-associated conditions affect tissue exposure to circulating material.
These experimental systems allow researchers to examine interactions between the vessel wall, circulating immune cells, pathogens, and microbial products. They can be used to investigate vascular permeability, leukocyte trafficking, and endothelial responses under inflammatory conditions. The resulting observations support analysis of how infection-related vascular changes contribute to barrier dysfunction and tissue injury.
Researchers can assess whether experimental conditions alter barrier permeability, endothelial integrity, leukocyte adhesion, or leukocyte transmigration. They can also examine interactions between circulating pathogens or microbial products and the endothelial layer. Together, these outcomes distinguish changes in barrier function from changes in immune-cell movement and help characterize vascular inflammation during infection.