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A Real-time Electrical Impedance Based Technique to Measure Invasion of Endothelial Cell Monolayer by Cancer Cells
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Medicine
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JoVE Journal Medicine
A Real-time Electrical Impedance Based Technique to Measure Invasion of Endothelial Cell Monolayer by Cancer Cells

A Real-time Electrical Impedance Based Technique to Measure Invasion of Endothelial Cell Monolayer by Cancer Cells

DOI:
10.3791/2792-v

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08:51 min

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April 01, 2011

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,
1Lombardi Comprehensive Cancer Center,Georgetown University

Chapters

  • 00:05Title
  • 01:46Preparation
  • 03:56Generating HUVEC Monolayer
  • 05:52Addition of Invading Cells and Data Normalization
  • 07:27Representative Invasion Assay Results
  • 08:18Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes an in vitro technique for monitoring cancer cells invading through a monolayer of endothelial cells. The data is acquired in real-time as a function of changes in impedance on the surface of electrodes at the well bottom.

Tags

Real-timeElectrical ImpedanceTechniqueMeasureInvasionEndothelial Cell MonolayerCancer CellsMetastatic DisseminationBasement Membrane DegradationTumor Cell AttachmentVascular EndotheliumEndothelial Junction RetractionInvasion And MigrationCirculatory SystemAdherence To Capillary WallsMetastatic Tumors FormationTumor Cell-endothelial Cell InteractionIn Vitro ReplicationMonolayer Of Human Umbilical Vein Endothelial Cells (HUVEC)Cancer Cell ChallengeElectron MicroscopyPhase-contrast MicroscopyIn Vivo Metastatic Process RepresentationElectrical Impedance Fluctuations MeasurementXCELLigence InstrumentRoche Manufacturing

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