Ivis System
Ivis System - Related Videos
08:53
In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum
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Cited by 58 •
2009
Mammary tumor cells expressing luciferase are implanted subcutaneously in mice and visualized using optical imaging to monitor tumor growth and development non-invasively in a longitudinal study.
10:21
In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus
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Cited by 11 •
2012
Utilizing luciferase and in vivo imaging systems (IVIS) as a novel means to identify disease endpoints before clinical developments occur. IVIS has allowed us to visualize in real time the invasion of encephalitic viruses over multiple days, providing a more accurate disease model for future study. It has also allowed us to identify the potential protective features of antivirals and vaccines faster than currently utilized animal models. The capability to utilize individual animals over...
09:53
In vivo Imaging of Transgenic Leishmania Parasites in a Live Host
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Cited by 65 •
2010
An in vivo imaging system is used to generate quantitative measurements of murine infection with the Trypanosomatid protozoan Leishmania. This is a non-invasive and non-lethal method for detecting parasites expressing luciferase within many tissues throughout the course of chronic Leishmania spp. infection.
08:44
Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
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Cited by 7 •
2013
Genes can be manipulated during development of the cortex or the hippocampus of the rat via in utero electroporation (IUE) at E16, to enable fast and targeted modifications in neuronal connectivity for later studies of behavior or neuropathology in adult animals. Postnatal in vivo imaging for control of IUE success is performed by bioluminescence of activating co-transfected luciferase.
11:22
A Standardized Protocol for Rearing Carausius morosus (Phasmatodea, Insecta) in Laboratory Settings
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2026
This paper outlines detailed protocols for maintaining a Carausius morosus laboratory colony, including key procedures for animal husbandry, egg handling, hatchling care, representative performance metrics, and an overview of the species lifespan and biological characteristics.
FAQs
A sensitive cooled camera detects light emitted by infected cells, pathogens, or engineered reporter cells and converts the signal into spatial maps of light intensity. The location of the signal helps indicate where biological activity is occurring, while changes in intensity or distribution across repeated measurements can be related to disease progression, pathogen dissemination, or treatment response.
The signal may originate from infected cells, the pathogen itself, or engineered reporter cells, depending on the experimental design. Bioluminescent and fluorescent signals provide the optical readout that the camera measures. Identifying the emitting biological component is important because the resulting map must be interpreted in relation to the process being studied, such as infection, inflammation, or immune-cell movement.
Spatial distribution shows where activity is concentrated, whereas light intensity provides a quantitative measure for comparing biological activity over time. Considering both features can reveal whether infection is spreading, inflammation is changing location, or immune-cell trafficking is occurring. These measurements help connect optical observations with disease progression, host-pathogen interactions, and responses to therapy.
An experiment first uses infected cells, pathogens, or engineered reporter cells capable of producing a detectable optical signal. The subject is then imaged with the sensitive cooled camera, which generates maps of signal location and intensity. Repeating this imaging over time allows researchers to follow changes in dissemination, inflammation, immune responses, or treatment effects within the same animal.
Longitudinal imaging follows biological changes in one animal across multiple time points rather than relying only on separate endpoint samples. This approach can show how infection or an immune response develops, changes, and responds to treatment over time. It also reduces the need for endpoint sampling, making temporal patterns easier to associate with disease progression or therapeutic efficacy.
The platform can support studies of pathogen dissemination, inflammation, immune-cell trafficking, treatment responses, and host-pathogen interactions. Researchers can examine how signal distribution changes during disease and whether treatment alters the observed activity. Because the same subject can be monitored repeatedly, the system is particularly relevant when the timing and progression of an immune or infectious process are important outcomes.