Nss Gene Manipulation
Nss Gene Manipulation - Related Videos
09:13
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
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Cited by 33 •
2011
The reverse genetics system for the Rift Valley fever virus MP-12 vaccine strain is a useful tool for creating additional MP-12 mutants with increased attenuation and immunogenicity. We describe the protocol to generate and characterize NSs mutant strains.
10:39
Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish
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Cited by 3 •
2017
An optimized protocol for the in vitro maturation of zebrafish oocytes used for the manipulation of maternal gene products is presented here.
07:01
Manipulation of Gene Function in Mexican Cavefish
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Cited by 42 •
2019
We describe approaches for the manipulation of genes in the evolutionary model system Astyanax mexicanus. Three different techniques are described: Tol2-mediated transgenesis, targeted manipulation of the genome using CRISPR/Cas9, and knockdown of expression using morpholinos. These tools should facilitate the direct investigation of genes underlying the variation between surface- and cave-dwelling forms.
13:27
Osteopathic Manipulative Treatment as a Useful Adjunctive Tool for Pneumonia
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Cited by 50 •
2014
Pneumonia is one of the top ten leading causes of death in the U.S. Osteopathic manipulative techniques (OMT) may be utilized as an adjunctive therapy in the treatment of pneumonia to enhance biomechanical and immune function; and ultimately, to reduce hospital stay, duration of antibiotics, incidence of respiratory failure, and mortality. In this video-article, we will review randomized controlled studies on the use of OMT in pneumonia patients, as well as demonstrate specific hands-on...
04:15
Stereotactic Microinjection of a Viral Vector for Gene Manipulation in a Mouse Pup Striatum
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2025
Source: Chen, S., et al., Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains. J. Vis. Exp. (2018)This video demonstrates the stereotaxic injection of a viral vector encoding Cre recombinase and green fluorescence protein or GFP into the striatum of a genetically modified mouse pup to achieve gene manipulation by excising a loxP-flanked STOP cassette, activating red fluorescence protein or RFP as a reporter.
FAQs
Comparison with an unmodified control helps link a change in pathogen behavior to the engineered Nss variant rather than to unrelated experimental differences. Researchers can examine replication, protein expression, and interactions with infected cells in parallel. This controlled comparison strengthens conclusions about how the Nss-encoded product contributes to pathogen biology or host responses.
Alteration, deletion, and replacement create defined genetic differences that can be evaluated for distinct effects. Comparing these variants shows whether the Nss-dependent phenotype changes when the gene or its sequence is modified. The approach therefore connects a specific genetic feature with outcomes such as replication, protein expression, or interactions between the pathogen and infected cells.
In immunology and infection research, the approach can reveal whether Nss-dependent effects involve innate immune signaling, immune evasion, or pathogenicity. Measuring how defined variants influence infected-cell interactions helps distinguish effects on the pathogen from effects on host responses. These findings can expose how an Nss product shapes infection and immune detection.
Molecular cloning, site-directed mutagenesis, and reverse-genetics systems are the principal strategies identified for generating Nss variants. The selected approach can produce a targeted alteration, deletion, or replacement that is then compared with an unmodified control. Using defined genetic constructs allows subsequent differences in pathogen and host-related measurements to be interpreted in relation to the Nss gene.
Key comparisons include pathogen replication, expression of the relevant protein, and interactions with infected cells. Together, these measurements provide complementary information: replication reflects pathogen performance, protein expression indicates an effect on the encoded product, and infected-cell interactions connect the genetic change to host responses. Reviewing all three can clarify the biological significance of an Nss-dependent effect.
Defined Nss mutants can clarify virulence mechanisms by showing how the gene contributes to pathogenicity or immune evasion. If the encoded product produces a measurable infection-related effect, that function may also identify a potential therapeutic target. The same experimental knowledge can support development of attenuated strains or improved models for studying infection and host responses.