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Manipulation of Gene Function in Mexican Cavefish

Manipulation of Gene Function in Mexican Cavefish

Article DOI: 10.3791/59093-v 07:01 min April 22nd, 2019
April 22nd, 2019



We describe approaches for the manipulation of genes in the evolutionary model system Astyanax mexicanus. Three different techniques are described: Tol2-mediated transgenesis, targeted manipulation of the genome using CRISPR/Cas9, and knockdown of expression using morpholinos. These tools should facilitate the direct investigation of genes underlying the variation between surface- and cave-dwelling forms.



Gene Function Manipulation Mexican Cavefish Genetic Tools Surface Fish Cave Fish Phenotypes Astyanax Mexicanus Postdoc Egg Injection Mold Agarose Needle Puller Glass Pipette Single Cell Eggs Morpholino Injection
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