Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

Manipulation of Gene Function in Mexican Cavefish
 

Manipulation of Gene Function in Mexican Cavefish

Article doi: 10.3791/59093
April 22nd, 2019

Summary April 22nd, 2019

We describe approaches for the manipulation of genes in the evolutionary model system Astyanax mexicanus. Three different techniques are described: Tol2-mediated transgenesis, targeted manipulation of the genome using CRISPR/Cas9, and knockdown of expression using morpholinos. These tools should facilitate the direct investigation of genes underlying the variation between surface- and cave-dwelling forms.

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter