Vaginal Lavage
Vaginal Lavage - Related Videos
09:01
Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification
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Cited by 673 •
2012
Here, we describe how to identify the stage of the murine reproductive (proestrus, estrus, metestrus, or diestrus) by simple, non-invasive collection and cytological assessment of vaginal smear samples. We further describe how vaginal cytology reflects circulating hormonal levels underlying transition through the murine reproductive cycle.
07:57
Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis
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Cited by 83 •
2011
Key techniques to be used in the evaluation of Candida vaginitis in an experimental animal model are described. The methods will allow rapid collection of vaginal specimens and lymphocytes from draining lumbar lymph nodes. These techniques could give rise to mouse models of other diseases in the female lower genital tract.
02:58
Modeling Murine Vaginal Colonization by Anaerobic Bacteria
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2025
Source: Morrill, S. R., et al. Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria. J. Vis. Exp. (2022)This video demonstrates a protocol for modeling vaginal colonization in mice using antibiotic-resistant anaerobic bacteria. It begins with hormonal treatment to increase epithelial susceptibility, followed by vaginal inoculation and co-inoculation under anaerobic conditions. The procedure concludes with post-inoculation sampling and culture to evaluate bacterial colonization...
10:19
A Murine Model of Group B Streptococcus Vaginal Colonization
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Cited by 64 •
2016
The purpose of this protocol is to imitate human group B Streptococcus (GBS) vaginal colonization in a murine model. This method may be used to investigate host immune responses and bacterial factors contributing to GBS vaginal persistence, as well as to test therapeutic strategies.
09:05
Rodent Estrous Cycle Monitoring Utilizing Vaginal Lavage: No Such Thing As a Normal Cycle
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Cited by 16 •
2021
This study details the crucial factors to consider in experimental designs involving female rats. In a larger sense, these data serve to decrease stigma and assist in the development of more inclusive diagnostic and intervention tools.
FAQs
Vaginal lavage captures more than microorganisms: the recovered fluid can contain cells, soluble molecules, and microbial material from the local mucosal environment. Laboratory analysis therefore permits parallel examination of cellular changes, immune mediators, inflammatory signals, and pathogen-associated components. This combination helps connect local host responses with infection-related biological changes.
Because the method does not require tissue removal, researchers can assess local responses repeatedly during an investigation. Sequential samples may help track changes associated with pathogen colonization, disease progression, reproductive health, or an intervention. This temporal perspective is especially valuable when the goal is to relate changing immune or inflammatory signals to infection-related processes.
The technique provides access to the local mucosal environment where host responses and microorganisms interact. Investigators can examine immune mediators, inflammatory signals, microbial components, and cellular changes within recovered fluid. These measurements help characterize mucosal immunity and clarify how local biological responses relate to infection, colonization, or reproductive health.
A typical collection introduces sterile fluid into the vaginal canal and then recovers that fluid by aspiration. The recovered material contains components released or present in the local environment and is transferred for laboratory analysis. This workflow samples the mucosa without removing tissue, allowing investigators to examine cells, soluble molecules, and microorganisms.
Researchers may select vaginal lavage when they need to evaluate local host responses, pathogen colonization, or disease progression through fluid-based samples. It can also support studies assessing vaccines or antimicrobial interventions. By examining immune mediators, inflammatory signals, microbial components, or cellular changes, investigators can compare local mucosal findings across infection-related conditions or study stages.
Analysis can identify patterns in immune mediators, inflammatory signals, microbial components, and cellular changes associated with infection or reproductive health. These findings may describe the local mucosal response, indicate microbial colonization, or show how that environment changes during disease progression. The same approach can help evaluate local effects of vaccines and antimicrobial interventions.