Luminex Assay
Luminex Assay - Related Videos
14:48
Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method
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Cited by 53 •
2012
An ELISA can be easily converted to a Luminex xMAP assay and, through the benefits of multiplexing, several antibodies can be screened simultaneously to identify an optimum antibody pair, resulting in increased sensitivity and dynamic range, while reducing assay cost.
12:56
An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry
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Cited by 10 •
2019
The in vitro micronucleus assay is a well-established method for evaluating genotoxicity and cytotoxicity but scoring the assay using manual microscopy is laborious and suffers from subjectivity and inter-scorer variability. This paper describes the protocol developed to perform a fully automated version of the assay using multispectral imaging flow cytometry.
09:11
Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence
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Cited by 5 •
2023
The micronucleus (MN) assay is a well-established test for quantifying DNA damage. However, scoring the assay using conventional techniques such as manual microscopy or feature-based image analysis is laborious and challenging. This paper describes the methodology to develop an artificial intelligence model to score the MN assay using imaging flow cytometry data.
06:29
Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay
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Cited by 9 •
2011
We describe a simple immunoassay to measure the production of pro-inflammatory cytokines, such as IL-1 beta production, in patients presenting with autoinflammatory phenotypes. By activating cells in whole blood cultures with pathogen-associated molecular patterns, specifically with lipopolysaccharide, cytokine secretion can be conveniently evaluated in whole blood supernatants.
08:12
The TUNEL Assay
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2023
One of the hallmarks of apoptosis is the nuclear DNA fragmentation by nucleases. These enzymes are activated by caspases, the family of proteins that execute the cell death program. TUNEL assay is a method that takes advantage of this feature to detect apoptotic cells. In this assay, an enzyme called terminal deoxynucleotidyl transferase catalyzes the addition of dUTP nucleotides to the free 3’ ends of fragmented DNA. By using dUTPs that are labeled with chemical tags that can produce...
FAQs
Each microsphere population carries a distinct fluorescent signature associated with a particular capture target. After the sample interacts with the beads, the analyzer first identifies the bead type and therefore the target being measured. This coding allows several analytes to be distinguished within the same sample rather than combining their signals into one undifferentiated measurement.
A labeled detection molecule produces a signal after the target is captured on its corresponding bead. Signal intensity reflects the amount of that analyte present, while the analyzer links the intensity to the bead’s identity. The resulting combination of bead classification and signal strength provides a separate measurement for each target included in the assay.
Multiplexing makes it possible to examine several proteins or other analytes from a single sample. This is particularly useful when a biological response contains multiple interacting signals, because the assay can profile them together while conserving sample material. The approach therefore supports broader characterization of coordinated changes than a measurement focused on only one analyte.
Neuroscience studies can apply the assay to cerebrospinal fluid, blood, or tissue extracts. Relevant targets include cytokines, chemokines, growth factors, and disease-associated proteins. Measuring these groups in different biological materials enables researchers to examine molecular signals associated with neural signaling, neuroinflammation, neurodegeneration, or responses to treatment.
By measuring multiple cytokines, chemokines, growth factors, or disease-associated proteins together, the assay helps characterize molecular profiles linked to neuroinflammation and neurodegeneration. These profiles can reveal patterns across several analytes rather than emphasizing a single marker. Researchers can use that broader information to investigate complex disease-associated responses in relevant samples.
The method is useful when treatment effects may involve several molecular signals rather than one protein alone. Researchers can assess groups of analytes in cerebrospinal fluid, blood, or tissue extracts and examine the resulting profile as part of a treatment-response study. Its sample-sparing, multiplexed design supports this type of broad molecular assessment when sample availability matters.