Mouse Skin Sections
Mouse Skin Sections - Related Videos
08:31
Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin
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Cited by 14 •
2017
This work presents a novel processing and imaging protocol for thick, three-dimensional tissue cross-section analysis that enables the full exploitation of confocal imaging modalities. This protocol preserves antigenicity and represents a robust system to analyze skin histology and potentially other tissue types.
11:13
Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
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Cited by 19 •
2015
The indirect immunofluorescence protocol described in this article allows the detection and the localization of proteins in the mouse mammary gland. A complete method is given to prepare mammary gland samples, to perform immunohistochemistry, to image the tissue sections by fluorescence microscopy, and to reconstruct images.
11:27
Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
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Cited by 2 •
2008
We describe a reliable method for immunolocalization within the epidermis modified for both frozen and paraffin sections that we use very routinely in our laboratory.
03:35
A Technique for Human Skin Grafting in a Mouse Model
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2025
This video demonstrates a method for grafting human skin onto an immunodeficient mouse with a suppressed neutrophil response. The method involves applying human split-thickness skin to the mouse's graft bed, sealing it, and covering it for recovery. Eventually, the human skin adheres to the mouse's skin, establishing vascularization and circulation, resulting in a successful skin xenograft.
04:48
Preparation of Mouse Vomeronasal Organ for Vibratome Sectioning
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2025
This video outlines the isolation of a mouse vomeronasal organ (VNO) and tissue preparation for vibratome sectioning. Sections are used to characterize the biophysical and physiological attributes of a specific group of sensory neurons.
FAQs
Fixation preserves the original tissue structure, while embedding provides the support needed to cut skin into thin, consistent slices. Together, these steps help maintain relationships among the epidermis, dermis, sensory nerve fibers, and associated cells during sectioning. Preserving those relationships is essential when microscopy is used to compare tissue organization or neural features across experimental conditions.
Serial sections provide a sequence of adjacent tissue slices rather than a single isolated view. Examining that sequence can support assessment of sensory nerve fibers and their distribution through different parts of the skin. In neuroscience experiments, this organized sampling helps researchers compare structural patterns and identify changes associated with altered sensation, pain, injury, or disease.
Staining or labeling distinguishes tissue structures and selected cellular or neural features that may not be readily separable by tissue appearance alone. In these sections, such approaches can help identify the epidermis, dermis, sensory nerve fibers, and associated cells. The resulting visual contrast supports evaluation of cutaneous innervation and neuroimmune relationships in defined experimental conditions.
Preparation generally proceeds by fixing the skin, embedding it in a supportive medium, cutting serial sections, and then staining or labeling the slices. Each stage serves a different purpose: preservation, mechanical support, organized sampling, and feature discrimination. The completed sections can then be examined microscopically for tissue architecture, cellular features, and neural structures.
Researchers can use them when a study requires structural assessment of skin innervation or interactions between neural and immune-associated cells. They are relevant to investigations of pain, sensation, injury, and disease because microscopy can reveal tissue or neural changes linked to those conditions. Comparisons across experimental groups can connect visible structural differences with the study design.
Microscopy can support comparisons of epidermal and dermal organization, sensory nerve fiber features, and associated cellular patterns between experimental conditions. These observations may indicate whether cutaneous innervation or neuroimmune relationships change in connection with pain, altered sensation, injury, or disease. Interpretation depends on examining the prepared sections consistently across the conditions being compared.