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In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

Article DOI: 10.3791/1958-v • 09:20 min • July 23rd, 2010
July 23rd, 2010 •
1, 1, 1, 2, 1
1Department of Biochemistry, School of Medical Sciences, University of Bristol, 2Division of Immunity and Infection, School of Medicine, University of Birmingham

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In situ subcellular fractionation of mammalian cells on microscope coverslips allows the visualisation of protein localisation.

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In Situ Subcellular Fractionation Adherent Mammalian Cells Non-adherent Mammalian Cells Protein Localization Protein Compartmentalization Protein Sub-populations Specific Structures Transcription Factors Chromatin Association Nuclear Scaffold Nuclear Matrix Microscope Coverslips Protein Visualization Antibodies Fluorescent Fusion Proteins Fluorescence Microscopy Specific Compartments Or Structures Protein Mapping Western Blotting
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