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Identifikation af protein-komplekser i
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Identifikation af protein-komplekser i

Article DOI: 10.3791/4057-v • 14:58 min • November 12th, 2012
November 12th, 2012 •
1,2, 1, 1, 1,3, 1,3
1Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, 2Deparment of Biochemistry, Research and Innovation Centre, University of Regina, 3Department of Medical Genetics and Microbiology, University of Toronto

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Affinitetsoprensning af mærkede proteiner i kombination med massespektrometri (APMS) er en kraftig fremgangsmåde til systematisk kortlægning af protein-interaktion net og til undersøgelse af den mekanistiske basis af biologiske processer. Her beskriver vi en optimeret sekventiel peptid affinitet (SPA) APMS fremgangsmåde udviklet til bakterien

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Genetik molekylærbiologi medicin biokemi mikrobiologi affinitetsoprensnings, Gram-negative bakterier cytosoliske proteiner SPA-tagging homolog rekombination massespektrometri protein-interaktion proteinkompleks
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