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Metabole Labeling van leucine rich repeat Kinasen 1 en 2 met radioactieve fosfaat

Article DOI: 10.3791/50523-v • 11:31 min • September 18th, 2013
September 18th, 2013 •
1, 1, 1
1Laboratory for Neurobiology and Gene Therapy, Department of Neurosciences, KU Leuven and Leuven Institute for Neuroscience and Disease (LIND)

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Leucine rich repeat kinases 1 en 2 (LRRK1 en LRRK2) zijn multidomain eiwitten die zowel GTPase en kinase domeinen coderen en die gefosforyleerd zijn in cellen. Hier presenteren we een protocol om LRRK1 en LRRK2 label in cellen met 32p orthofosfaat, waardoor een middel om hun algemene cellulaire fosforylering te meten.

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Cellular Biology biologie (algemeen) biochemie bio-ingenieur (algemeen) LRRK1 LRRK2 metabole etikettering 32P orthofosfaat immunoprecipitatie autoradiografie
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