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Hurtig isolering og oprensning af mitokondrier til transplantation Ved Tissue Dissociation og differentieret Filtrering
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Hurtig isolering og oprensning af mitokondrier til transplantation Ved Tissue Dissociation og differentieret Filtrering

Article DOI: 10.3791/51682-v • 10:35 min • September 6th, 2014
September 6th, 2014 •
1, 2, 1, 2, 2, 1
1Division of Cardiothoracic Surgery, Beth Israel Deaconess Medical Center and Harvard Medical School, 2Department of Anesthesiology, Perioperative and Pain Medicine, Boston Children's Hospital and Department of Anesthesia, Harvard Medical School

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En metode til hurtig isolering af mitokondrier fra pattedyr vævsbiopsier beskrives. Rotte lever eller skeletmuskulaturen præparater blev homogeniseret med en kommerciel væv dissociator og mitokondrier blev isoleret ved differential filtrering gennem nylon mesh filtre. Mitokondrie isolation tid er <30 min sammenlignet med 60-100 minutter ved hjælp af alternative metoder.

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Cellebiologi kirurgiske procedurer operative efterforskningsteknikker mitokondrier isolering filtrering homogenatet væv dissociation transplantation
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