Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / In Vivo Microinjection and Electroporation of…
JoVE Journal
Biology

Se requiere una suscripción a JoVE para ver este contenido.

JoVE Journal Biology
In Vivo Microinjection and Electroporation of Mouse Testis
Operation of Laboratory Photobioreactors with Online Growth Measurements and Customizable Light Regimes
Journal (Biology)
Operation of Laboratory Photobioreactors with Online Growth Measurements and Customizable Light Regimes
Drug Screening Using Peripheral Blood Mononuclear Cells (PBMCs) and Cell Lysate Preparation for mRNA Sequencing (Video) | JoVE
Journal (Biology)
Drug Screening Using Peripheral Blood Mononuclear Cells (PBMCs) and Cell Lysate Preparation for mRNA Sequencing (Video) | JoVE
 

In Vivo Microinjection and Electroporation of Mouse Testis

Article DOI: 10.3791/51802-v • 08:39 min • August 23rd, 2014
August 23rd, 2014 •
1, 1, 1
1Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN)

Capítulos

Resumen
Automatic Translation

This article describes microinjection and electroporation of mouse testis in vivo as a transfection technique for testicular mouse cells to study unique processes of spermatogenesis. The presented protocol involves steps of glass capillary preparation, microinjection via the efferent duct, and transfection by electroporation.

Tags

Microinjection Electroporation Mouse Testis Transfection Technique Gene Transfer Reporter Plasmid Constructs EGFP DsRed2 CMV Promoter Anaesthetized Animal Efferent Duct Rete Testis Seminiferous Tubules Electroporation DNA Solution
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter