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Sequentie-specifieke Labeling van nucleïnezuren en eiwitten met methyltransferases en Cofactor Analogues

Article DOI: 10.3791/52014-v • 12:07 min • November 22nd, 2014
November 22nd, 2014 •
*1, *1, 1, 1, 1
1Institute of Organic Chemistry, Department of Chemistry, RWTH Aachen University
* These authors contributed equally

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DNA en eiwitten sequentie-specifiek gemerkt met affiniteit of fluorescente reporter groepen met DNA of eiwit methyltransferases en synthetische analogen cofactor. Afhankelijk van de cofactor specificiteit van de enzymen, aziridine of dubbel geactiveerde cofactor analogen worden gebruikt voor één of twee stappen labeling.

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Biochemie , AdoMet SAM aziridine- cofactor dubbele geactiveerd cofactor methyltransferase DNA-methylatie eiwit methylatie biotine etikettering fluorescentielabeling glimlachen mTAG
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