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Imaging Ca<sup> 2+</sup> Dynamics i Cone fotoreseptor Axon terminaler Mouse Retina
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1Institute for Ophthalmic Research,University of Tübingen, 2Graduate School of Cellular & Molecular Neuroscience,University of Tübingen, 3Bernstein Centre for Computational Neuroscience,University of Tübingen, 4Molecular Genetics Laboratory,University of Tübingen, 5Centre for Ophthalmology,University of Tübingen
Capítulos
- 00:05Título
- 01:59Eye Enucleation and Isolation of Retina
- 03:22Slice Preparation
- 04:59Two-photon Microscopy
- 06:14Light Stimulation and Recording
- 06:53Results: Light-evoked Ca2+ Responses in Axon Terminals of Mouse Cone Photoreceptors
- 08:10Conclusion
Vi beskriver en protokoll for å overvåke Ca 2 + dynamikk i axon terminalene kjegle fotoreseptorer under anvendelse av en ex-vivo skive fremstilling av musen hinnen. Denne protokollen tillater omfattende studier av kjegle Ca 2+ signale i en viktig pattedyr modellsystem, musen.
