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Anvender digitale Droplet PCR til Detect BRAF V600E Mutationer i formalinfikserede paraffinindlejrede Reference Standard cellelinier

Article DOI: 10.3791/53190-v • 10:16 min • October 8th, 2015
October 8th, 2015 •
1, 3, 1, 3, 4, 2, 2, 1,2
1Research Institute of Pharmaceutical Science, Department of Pharmacy, College of Pharmacy, Seoul National University, 2The Center for Anti-Cancer CDx, N-Bio, Seoul National University, 3ABION CRO, 4ABION Inc., R&D Center

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Målet med denne video er at vise, hvordan du udfører automatiseret DNA-ekstraktion fra formalin-fikseret paraffinindlejrede (FFPE) referencestandard cellelinjer og digital dråbe PCR (ddPCR) analyse til påvisning af sjældne mutationer i et klinisk miljø. Detektion af mutationer i FFPE prøver viser den kliniske anvendelighed af ddPCR i FFPE prøver.

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Molekylær Biologi DNA Mutation BRAF ddPCR molekylær biologi Fluorescens FFPE Kræftens biologi
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