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单元位置的Imaging-和流式细胞仪为基础的分析和3D球体黑色素瘤细胞周期
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Medicina
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JoVE Revista Medicina
Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids
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单元位置的Imaging-和流式细胞仪为基础的分析和3D球体黑色素瘤细胞周期

DOI:
10.3791/53486-v

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10:44 min

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December 28, 2015

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, , , ,
1The Centenary Institute, 2Sydney Medical School,University of Sydney, 3The University of Queensland Diamantina Institute, Translational Research Institute,The University of Queensland, 4Department of Dermatology,Royal Prince Alfred Hospital, 5Discipline of Dermatology,University of Sydney

Capítulos

  • 00:05Título
  • 01:063D Spheroid Formation
  • 03:30Image Analysis of FUCCI Spheroid Sections
  • 08:40Results: Quantification of G1 Arrested and Proliferating Cells in Cancer Cell Spheroids
  • 10:00Conclusion

Summary

Traducción Automática

Traducción Automática

We describe two complementary methods using the fluorescence ubiquitination cell cycle indicator (FUCCI) and image analysis or flow cytometry to identify and isolate cells in the inner G1 arrested and outer proliferating regions of 3D spheroids.

Tags

3D Tumor SpheroidsMelanomaCell CycleFUCCIImagingFlow CytometryCell PositionTumor MicroenvironmentHypoxiaDrug PenetrationNecrotic CoreProliferationCell-cell Interaction

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