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Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids
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Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids

Article DOI: 10.3791/53486-v • 10:44 min • December 28th, 2015
December 28th, 2015 •
1,2, 1,2, 3, *1,4,5, *1,3,5
1The Centenary Institute, 2Sydney Medical School, University of Sydney, 3The University of Queensland Diamantina Institute, Translational Research Institute, The University of Queensland, 4Department of Dermatology, Royal Prince Alfred Hospital, 5Discipline of Dermatology, University of Sydney
* These authors contributed equally

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résumé
Automatic Translation

We describe two complementary methods using the fluorescence ubiquitination cell cycle indicator (FUCCI) and image analysis or flow cytometry to identify and isolate cells in the inner G1 arrested and outer proliferating regions of 3D spheroids.

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Imaging Flow Cytometry Cell Position Analysis Cell Cycle Analysis 3D Melanoma Spheroids Tumor Microenvironment Cell-cell Interaction Hypoxia Nutrient Deprivation Drug Penetration Necrotic Core G1 Arrested Cells Proliferating Cells Drug Therapies Genetic Manipulation Environmental Manipulation Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) System Cytometry Image Analysis Software Cell Cycle Status Gene Expression Protein Expression Cell Viability
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